Tuberculosis and Respiratory Diseases
- Volume 41 Issue 3
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- Pages.248-261
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- 1994
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- 1738-3536(pISSN)
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- 2005-6184(eISSN)
Characterization and Distribution of Glycoconjugates in Human Pulmonary Tubercles by Lectin Histochemistry
폐결핵 결절에서 복합당질의 분포에 관한 Lectin 조직화학적 연구
- Yoon, Sik (Department of Anatomy, College of Medicine, Dong-A University) ;
- Kim, Ji-Hong (National Masan Tuberculosis Hospital) ;
- Shin, Cheol-Shik (National Masan Tuberculosis Hospital) ;
- Jeong, Suk (National Masan Tuberculosis Hospital) ;
- Son, Mal-Hyun (National Masan Tuberculosis Hospital) ;
- Song, Sun-Dae (National Masan Tuberculosis Hospital) ;
- Kim, Jin-Jeong (Department of Anatomy, College of Medicine, Pusan National University)
- 윤식 (동아대학교 의과대학 해부학교실) ;
- 김지홍 (국립 마산 결핵 병원) ;
- 신철식 (국립 마산 결핵 병원) ;
- 정숙 (국립 마산 결핵 병원) ;
- 손말현 (국립 마산 결핵 병원) ;
- 송선대 (국립 마산 결핵 병원) ;
- 김진정 (부산대학교 의과대학 해부학교실)
- Published : 1994.06.30
Abstract
Background: Lectins are proteins or glycoproteins of non-immune origin that recognize a specific sequence of sugar residues. The availability of a large number of lectins has provided the capacity to identify selectively glycoconjugates possessing distinctive chemical structure in diverse sites of highly specialized biological activity. The purpose of the present study was to investigate the lectin binding patterns of various components in human pulmonary tubercles. Method: Biopsy specimens of tuberculous lung were obtained from male adult patients who underwent a surgical resection for severe pulmonary tuberculosis. The specimens were processed and stained with 13 kinds of biotinylated lectins according to some modification of Hsu and Raine's methods. Results: 1) In the caseous necrotic lesions, BS
연구배경 : Lectin은 탄수화물의 특정한 당잔기에 높은 친화성과 특이성을 가지므로 이러한 lectin-sugar 상호작용을 이용하여 사람 폐결핵결절에서 이를 구성하는 주된 성분인 건락성 괴사 상피양세포, 림프구 및 섬유층에 존재하는 탄수화물의 종류와 분포에 대해 알아보고자, 본 연구를 실시하였다. 방법 : 사람 폐결핵 절제 조직에서, 13종의 lectin을 사용하여 조직화학염색을 시행하였다. 결과: 1) 건락성 괴사부에서 BS
Keywords
- Lectin;
- Histochemistry;
- Caseous necrosis;
- Epithelioid cell;
- Carbohydrate;
- Glycoconjugate;
- Pulmonary tubercle