Tuberculosis and Respiratory Diseases
- 제41권2호
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- Pages.135-143
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- 1994
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- 1738-3536(pISSN)
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- 2005-6184(eISSN)
결핵성 늑막삼출과 비결핵성 늑막삼출에서의 가용성 Interleukin-2 수용체의 농도
Soluble Interleukin-2 Receptor(sIL-2R) Levels in Patients Tuberculous Pleurisy VS Nontuberculous Pleurisy
- Lim, Hyun-Oak (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Ham, Jong-Yeol (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Shim, Dae-Seok (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Hwang, Young-Sil (Department of Internal Medicine, College of Medicine, Gyeong Sang National University)
- 발행 : 1994.04.30
초록
연구목적 : 혈청 sIL-2R 농도는 T세포의 면역활성화가 관여되는 육아종성질환, 장기이식, 자가면역질환에서, 또한 혈액종양 및 림프세망내 피계종양등에서 증가 한다고 알려져있다. 최근 결핵환자에서도 질병의 활성화 정도에 따라 혈청 및 흉강 삼출액에서 sIL-2R 농도의 증가를 보고하고 있다. 임상에서 결핵성 흉강삼출과 악성 흉강삼출의 감별이 어려운 경우를 경험하게 되는데 흉강삼출액에서 sIL-2R 농도 측정이 이들 질환의 감별에 도움이 될수 있는지 알아보기 위해 늑막조직검사에서 결핵성으로 확인된 12명의 결핵성 흉강삼출액환자와 32명의 비결핵성 흉강 삼출액환자를 대상으로 혈청 및 흉강삼출액에서 sIL-2R 농도를 측정하였다. 방법 : 대상환자의 늑막삼출액을 원심분리하여 얻은 상층액과 동시에 채취한 혈청을
Background: The cell mediated immunity has an important role in the pathogenesis of tuberculosis. sIL-2R has been known as a sensitive marker of T lymphocyte activation Elevated serum levels of sIL-2R have been found in patients with lymphoproliferative disorders, organ transplantation, autoimmune diseases, and various granulomatous diseases. Elevated levels of sIL-2R have been also found in the serum and pleural fluid of the patients with tuberculosis. To evaluate the diagnostic value of sIL-2R in the differentiation of tuberculous pleurisy and nontuberculous pleurisy. We measured the level of sIL-2R in the sera and pleural fluids of 12 patients with tuberculous pleurisy and 32 patients with nontuberculous pleurisy. Method: Samples of pleural fluid and serum were centrifuged at 2500 rpm for 10 min to remove cell pellets. Soluble IL-2R was measured with a sandwitch enzyme immunoassay using the Cellfree(r) Interleukin-2 Receptor Test kit(T-cell science,Inc. Cambridge, MA). Results: The results obtained were as follows: 1) The sIL-2R level in pleural fluid of the patients with tuberculous pleurisy was higher than that of patients with nontuberculous pleurisy(P<0.005). 2) When the sIL-2R level above 5,000 u/ml in pleural fluid was used as the cut-off value to diagnose tuberculous pleurisy, it had a sensitivity of 84.6% and a specificity of 90.9%. 3) The sIL-2R level in the sera of the patients with tuberculous pleurisy was higher than that of patients with bacterial pleural effusions and normal control group(P<0.05) and there was no difference of levels compared with malignant pleural effusions and transudative pleural effusions(P>0.05). 4) In patients with tuberculous pleurisy, the mean concentration of sIL-2R in pleural fluid was higher than that in serum(P<0.005). Conclusion: These findings suggest that the measurement of elevated levels of pleural fluid sIL-2R in tuberculous pleurisy may be useful in the differential diagnosis between patients with tuberculous pleurisy and nontuberculous pleurisy.