Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Triploidy Induction in Olive Flounder, Paralichthys olivaceus

3배체 넙치 (Paralichthys olivaceus)의 유도에 관한 연구

  • KIM Dong Soo (Department of Aquaculture, National Fisheries University of Pusan) ;
  • JEONG Chag Hwa (RCOID, National Fisheries University of Pusan) ;
  • LEE Young-Don (Marine Research Institute, Cheju National University) ;
  • RHO Sum (Department of Aquaculture, Cheju National University)
  • 김동수 (부산수산대학교 양식학과) ;
  • 정창화 (부산수산대학교 해양산업개발연구소) ;
  • 이영돈 (제주대학교 해양연구소) ;
  • 노섬 (제주대학교 증식학과)
  • Published : 1994.02.01
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Abstract

Triploid fish were induced successfully in olive flounder (Paralichthys olivaceus) by cold shocking fertilized eggs 3 minutes post fertilization at $2^{\circ}C$ for 45 minutes. Percent incidence of triploid was $92.6\%$ in this treatment. Floating rate and fertilization rate of eggs were not significanlty different from that of diploid controls (P> 0.05). However, hatchability and abnormal larvae of triploids were significantly different from that of diploid controls (P< 0.05). Incidence of triploidy was confirmed by erythrocyte measurements and chromosome counts. The surface area of triploid erythrocytes and nucleus was 1.6 times larger than that of diploids. Diploids had 48 acrocentric chromosomes, while triploids had 72.

넙치의 수정란을 수정 3분 후 $2^{\circ}C$에서 45분간 저온 처리하여 $92.6\%$의 빈도로 3배체를 유도하였다 수정란의 부상율 및 수정율은 실험군 사이에 유의차가 없었다(P> 0.05). 그러나 부화율과 기형율은 2배체와 3배체가 유의차가 있게 나타났다P< 0.05). 3배체를 적혈구 세포크기 측정 및 염색체 분석으로 확인한 결과 세포의 표면적에서 3배체는 2배체에 비해 1.6배 증가되었고, 2배체의 염객체수는 48개의 acrocentric인 반면 3배체는 72개의 acrocentric 염색체로 나타났다.

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