Journal of Yeungnam Medical Science
- 제11권2호
- /
- Pages.293-302
- /
- 1994
- /
- 2799-8010(eISSN)
Imipramine이 배뇨근 세포의 수축성에 미치는 직접작용
Effect of Imipramine on the Contractility of Single Cells Isolated from Canine Detrusor
- Huh, Chan-Wook (Department of Pharmacology College of Medicine, Yeungnam University) ;
-
Lee, Kwang-Youn
(Department of Pharmacology College of Medicine, Yeungnam University) ;
- Kim, Won-Joon (Department of Pharmacology College of Medicine, Yeungnam University)
- 발행 : 1994.12.30
초록
유뇨증의 치료제로서 널리 사용되고 있는 imipramine의 작용기전에 관한 학설이 여러가지가 있으나 그 중 콜린성수용체 봉쇄작용을 관찰하기 위한 방법으로 평활근 세포를 분리배양하여 단일세포에 대한 acetylcholine의 수축작용과 이에 대한 imipramine의 길항작용을 atropine의 그것과 비교해 보기로 하였다. 개의 방광을 적출하여
The objective of this study was to establish a good methodology to isolate single smooth muscle cells that are alive and respond properly to pharmacological agents. Canine urinary bladders were employed as the source of single cells, and acetylcholine, atropine and imipramine were used as indicators of pharmacological responsiveness. Imipramine, an antidepressant drug exhibited the anticholinergic and calcium antagonizing properties on rat detrusor muscle. To establish a control value for a further experiment to elucidate the mechanism of action of imipramine on detrusor muscle, we measured the concentration-response of single cells to acetylcholine in the presesnce of imipramine by length of the cells and compared the result with the response in the presence of atropine. Tiny chops of smooth muscle taken from anesthetized canine urinary bladder were incubated in collagenase solution at