Korean Journal of Environmental Agriculture (한국환경농학회지)
- Volume 13 Issue 3
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- Pages.262-270
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- 1994
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- 1225-3537(pISSN)
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- 2233-4173(eISSN)
Development of Radioimmunoassay(RIA) for Residue Analysis with Endosulfan in Water and Carp(Cyprinus carpio L.)
잉어중 Endosulfan의 잔류분석(殘留分析)을 위한 Radioimmunoassay(RIA)의 개발(開發)
- Lee, Kang-Bong (College of Agriculture Chonnam National University) ;
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Shim, Jae-Han
(College of Agriculture Chonnam National University) ;
- Suh, Yong-Tack (College of Agriculture Chonnam National University)
- Published : 1994.12.30
Abstract
The established methods in the residue analysis of endosulfan require an extensive sample clean-up prior to quantification by relatively complex equipment. A radioimmunoassay(RIA) provides a simple procedure with theoretically higher sensitivity and specificity necessitating only a minimum of sample clean-up. Endosulfan-specific antibodies were developed in rabbits by using a bovine serum albumin(BSA) conjugate wherein the alcohol form of endosulfan was multiply bound to the protein via succinylation. Produced antibodies showed the high titers to endosulfan-BSA(1 : 32,000). An RIA method was developed in water and carp by using
Endosulfan의 잔류분석(殘留分析)을 위한 RIA(radioimmuno assay)는 endosulfan alcohol(EA)-BSA conjugate를 항원(抗原)으로 하여 집토끼에서 면역항체(免疫抗體)를 생산(生産)하였다. 생산(生産)된 면역항체(免疫抗體)의 역가(力價)는 1 : 32,000 으로 endosulfan과 EA 이외(以外)의 유사화합물(類似化合物)에서는 거의 반응성(反應性)을 나타내지 않았으며 RIA를 위한 최적(最適) 희석배수(稀釋倍數)는 1 : 2,000 으로 나타났다. RIA의 최적(最適) 배양온도(培養溫度)는
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