한국환경농학회지 (Korean Journal of Environmental Agriculture)
- 제13권1호
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- Pages.76-82
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- 1994
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- 1225-3537(pISSN)
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- 2233-4173(eISSN)
Metalaxyl의 다클론 항체생산과 최적 ELISA 조건
Production of Polycolonal Antibody and Optimum Conditions in ELISA for Metalaxyl
- Lee, Kang-Bong (Dept. of Agric. Chemistry, College of Agriculture, Chonnam University) ;
- Im, Geon-Jae (Institute of Agricultural Chemicals) ;
- Jung, Young-Ho (Institute of Agricultural Chemicals) ;
- Suh, Yong-Tack (Dept. of Agric. Chemistry, College of Agriculture, Chonnam University)
- 발행 : 1994.04.30
초록
Metalaxyl을 ELISA기법을 이용하여 분석하기 위하여 metalaxyl-HSA를 합성하고 이에 대한 항체를 생산하여 competitive indirect ELISA를 위한 최적조건을 조사하였다. Metalaxyl-protein conjugate는 metalaxyl을 가수분해시켜 생성된 metalaxyl acid에 단백질(HSA, OA)을 mixed anhydride방법이나 EDC 첨가방법으로 조제하였고 생성된 항체의 역가는 1:16,000으로 나타났다. Coating Ag의 최적농도는
A competitive indirect enzyme-linked immunosorbent assay(ELISA) was developed to detect and quantify levels of the fungicide metalaxyl in crops. Antiserum against metalaxyl was demonstrated in rabbits immunized with metalaxyl-human serum albumin(HSA) conjugate. Metalaxyl-protein conjugate was prepared by mixed anhydride and peptide coupling method with EDC. In this assay, metalaxyl-ovalbumin(OA) was
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