Analytical Science and Technology (분석과학)
- Volume 7 Issue 3
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- Pages.413-419
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- 1994
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
Microanalysis of Vecuronium Bromide in Biological Fluids by Spectrofluorimetry and HPLC-Fluorescence Detection
형광광도법 및 HPLC-형광검출법에 의한 생체시료 중의 Vecuronium bromide의 미량분석
- Ko, Yong-Seok (College of Pharmacy, Wonkwang University) ;
- Han, Sang-Soo (College of Pharmacy, Wonkwang University) ;
- Shin, Tae-Yong (Dept. of Pharmacy, Chonju Woosuk University) ;
-
An, Nyeon-Hyoung
(College of Pharmacy, Wonkwang University)
;
- Ock, Chi-Wan (College of Pharmacy, Wonkwang University)
- Received : 1994.07.18
- Published : 1994.09.25
Abstract
The determination of the neuromuscular blocking agents vecuronium bromide(VeBr) in biological fluids has been investigated. The method depends on the formation of insoluble red complex between vecuronium bromide and rose bengal in aqueous layer. The amount of vecuronium bromide was calculated from that of extracted rose bengal which was determined by spectrofluorimetry or HPLC/fluorescence detection method. It was possible to analyze VeBr in the range of
생체시료 중에 함유된 베크로늄브로마이드(VeBr)를 정량하기 위해 로즈벵갈(RB)과 이온쌍착물(ion-pair complex)을 형성시켜 유기용매층으로 이행시킨 다음 형광광도법 및 형광검출기를 이용한 고속액체크로마토그래프(HPLC)법으로 정량하였다. 이 때 이온쌍착물의 최적 추출조건을 검토하기 위하여 완충액의 pH, 추출용매, 진탕시간의 영향을 실험하였다. 한편, 이온착물의 형성 및 조성은 연속변화법, 몰비법, IR,