치과방사선 (Journal of Korean Academy of Oral and Maxillofacial Radiology)
- 제24권2호
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- Pages.249-260
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- 1994
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- 1225-049X(pISSN)
수종의 암세포주의 저선량 방사선감수성에 관한 실험적 연구
AN EXPERIMENTAL STUDY ON THE LOW-DOSE RADIOSENSITIVITY OF TUMOR CELL LINES
- Kim Min-Sook (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University) ;
- Koh Kwang-Joon (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University)
- 발행 : 1994.12.01
초록
The purpose of this study was to aid in the radiation therapy of head and neck cancer patients. For this study, radiation survival curves were generated for Bl6, MG-63 and YAC-l cell lines using semiautomated MTT assay and Dye Exclusion Assay. Irradiation of 2, 4, 6, 8, 10Gy were delivered at room temperature at a dose rate of 210.2cGy/min using / sup 60/Co γ-ray Irradiator ALOORAOO 8. The viable cells were determined for each radiation dose and compared to control values. The obtained results were as follows: 1. There was significantly different absorbance at 10Gy on B16 cell line in MTT assay(P<0.05). 2. There was significantly different absorbance at 4, 6, 8, 10Gy on MG-63 cell line in MTT assay(P<0.05). 3. YAC-l cell line was more sensitive than B16 or MG-63 cell line to all doses of radiation(P<0.05). 4. There was significantly different absorbance among all tumor cell lines except between B16 and MG-63 cell line at ZGy in MTT assay(P<0.05). 5. Good correlation was obtained between MTT assay and DEA(P<0.05). The efficient of correlation of B16, MG-63 and YAC-l cell line was 0.845, 0.824 and 0.906, respectively.
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