Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Growth and Rotting Characteristics of Lentinus edodes Isolates in Korea and Their Adaptability to Bag-Culture Using Oak Sawdust

우리나라 표고의 생장(生長), 부후특성(腐朽特性) 및 톱밥배양(培養)에 관(關)한 연구(硏究)

  • Bak, Won Chull (Dept. of Forest Microbiology, Forestry Research Institute) ;
  • Lee, Eun Young (Dept. of Forest Microbiology, Forestry Research Institute) ;
  • Yoon, Kab He (Dept. of Forest Microbiology, Forestry Research Institute) ;
  • Lee, Won Kyu (Dept. of Forest Microbiology, Forestry Research Institute) ;
  • Yi, Chang Keun (Dept. of Forest Microbiology, Forestry Research Institute) ;
  • Hong, Ki Sung (Dept. of Agricultural Biology, College of Agriculture, Chungbuk National University)
  • 박원철 (임업연구원 산림미생물과) ;
  • 이은영 (임업연구원 산림미생물과) ;
  • 윤갑희 (임업연구원 산림미생물과) ;
  • 이원규 (임업연구원 산림미생물과) ;
  • 이창근 (임업연구원 산림미생물과) ;
  • 홍기성 (충북대학교 농과대학 농생물학과)
  • Received : 1993.08.24
  • Published : 1994.03.31
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Abstract

Studies were made to find out the mycelial growth and rotting characteristics of 91 Lentinus edodes isolates collected or hybridized in Korea and abroad, and their adaptability to bag-culture. The results are as follows. 1. There were little differences in mycelial growth at PDA and oak (Quercus mongolica) sawdust-based medium, and rotting among high-, mid- and low temperature types of L. edodes isolates. But, the fruit yield of high-temperature type was much higher than those of the other two types. The fruit yields of the isolates in the same strain group were apparently different. 2. From the sawdust-based culture of 91 isolates, FRI 221, FRI 208 and FRI 169 were selected as excellent strains in yield and Quality, showing fruiting of 157g, 152g and 119g per 1kg-medium, respectively. 3. Attempts to culture in various media with different substrates resulted in almost same fruit yields, and the yield was proportional to medium weights as 2.5kg-medium showed 2.5 times more yield than 1kg-medium. Fruit yields were different according to incubation periods and the period of little more than 100days was best. 4. When the correlation analyses among mycelial growth, rotting ability, yield and fruit-shape normality were made with the 91 isolates, mycelial growth at sawdust-based medium showed highly positive correlation with that at PDA, while fruit yield was negatively correlated with mycelial growth in PDA and sawdust-based medium.

우리나라와 외국에서 수집(蒐集) 및 교잡(交雜)을 하여 얻은 91개의 표고(Lentinus edodes) 균주(菌株)를 공시(供試)하여 균사생장력(菌絲生長力), 부후력(腐朽力) 및 톱밥배지(培地)에서의 배양특성(培養特性) 등을 구명(究明)한 바, 그 결과는 다음과 같다. 1. 고온성(高溫性), 중온성(中溫性) 및 저온성(低溫性) 균주(菌株)의 PDA배지와 신갈나무(Quercus mongolica)톱밥배지(培地)에서의 균사생장력 및 부후력은 온도형간(溫度型間)에 별차이(別差異)가 없었으나, 톱밥배지에서의 버섯발생량(發生量)은 고온성 균주들이 월등(越等)히 높았다. 또한, 같은 계통(系統)에 속하는 균주들간에도 버섯발생량의 차이가 많은 것으로 나타났다. 2. 총 91개 균주의 톱밥재배 결과, FRI 221, FRI 208 및 FRI 169가 수확량(收穫量)과 품질(品質)이 우수(優秀)한 것으로 나타났으며, 1kg 배지당(培地當) 각각 157g, 152g 및 119g의 버섯 발생량을 보였다. 3. 톱밥배지의 조성(組成)을 여러 가지로 시도(試圖)한 바 버섯발생량에 영향(影響)을 주지 않았으며, 2.5kg배지에서의 발생량은 1kg배지의 약 2.5배를 보여 배지무게에 비례(比例)하였다. 또한, 배양기간(培養期間)에 따라 발생량이 달랐는데, 100여일(餘日)이 가장 좋았다. 4. 균사생장력, 부후력, 버섯발생량 및 버섯정형율간(正形率間)의 상관관계(相關關係)를 분석(分析)한 결과, PDA배지 및 생장력은 톱밥배지에서의 생장력과 정(正)의 상관(相關)을 보였으며, PDA 및 톱밥배지에서의 생장력은 버섯발생량과 부(負)의 상관(相關)을 나타냈다.

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