미생물학회지 (Korean Journal of Microbiology)
- 제32권2호
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- Pages.109-114
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- 1994
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
방향족 아미노산에 의한 대장균 serC-aroA Operon의 발현 억제
Repression of Escherichia coli serC-aroA Operon by Aromatic Amino Acids
- 황우길 (강원대학교 자연과학대학 생화학과) ;
- 사재훈 (강원대학교 자연과학대학 생화학과) ;
-
김경훈
(강원대학교 자연과학대학 생물학과)
;
-
임창진
(강원대학교 자연과학대학 생화학과)
- Hwang, Woo-Gil (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
- Sa, Jae-Hoon (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
-
Kim, Kyung-Hoon
(Department of Biology, College of Natural Sciences, Kangwon National University)
;
-
Lim, Chang-Jin
(Department of Biochemistry, College of Natural Sciences, Kangwon National University)
- 발행 : 1994.01.01
초록
대장균에서 두 가지 다른 아미노산의 생합성에 관여하는 serC 유전자와 araA유전자는 혼합 operon을 이루고 있다. SerC-aroA 혼합 operon의 발현 조절 현상을 serC-aroA-lacZ fusion plasmid pWH2를 이용하여 측정하였다. serC-aroA 혼합 operon의 발현은 L-tyrosine, L=phenulalanine 및 L-tryptophan 등 방향족 아미노산들에 의하여 억제되었다. 방향족 아미노산에 의한 억제 효과는
The Escherichia coli aroA and serC genes constitute a mixed-function operon which involves in two different amino acid biosynthetic pathways. The regulation of expression of serC-aroA operon was evaluated through the use of a serC-araA-lacZ fusion plasmid pWH2. The expression of the serC-aroA operon was decreased by aromatic amino acids such as tyrosine, tryptophan, and phenylalanine. The repressible effects were diminished in E. coli tyrR of trpR strain, indicating the involvemnt of TyrR of TrpR protein in the repression. Tyrosine was competitie with cAMP in the influence on the expression of the serC-AroA operon. From these data, it was suggested that the serC-aroA operon is controlled by aromatic amino acids in a negative manner.