DOI QR코드

DOI QR Code

Enzymatic Hydrolysis of p-Nitrophenyl Phsphoryl Derivatives by Phospholipase D

  • Cha, Joo-Yeun (Department of Chemsitry, Seoul National University) ;
  • Lee, Ji-Eun (Department of Chemsitry, Seoul National University) ;
  • Koh, Eun-Hie (Department of Chemistry, Duksung Women's University) ;
  • Choi, Myung-Un (Department of Chemsitry, Seoul National University)
  • 발행 : 1994.11.20

초록

A series of phosphodiesters of p-nitrophenyl phosphoryl derivatives were synthesized and used as a model substrate for phospholipase D (PLD). The phosphodiester substrates were synthesized from p-nitrophenyl phosphorodichloridate and corresponding alcohols with different chain lengths and polar groups. To measure the activity of PLD, either spectroscopic method for p-nitrophenol or pH-stat titration method was employed. For each substrate, effects of substrate concentration, pH, and $Ca^{2+}$ ion were examined. The kinetic parameters $V_{max}$ for the different substrates were varied depending on the chain lengths or charge of the alcohols. No calcium effect was observed in the hydrolysis of neutral and negatively charged alcohol derivatives, while positively charged choline derivative showed a strong $Ca^{2+}$ ion dependence.

키워드

참고문헌

  1. Adv. Lipid Res. v.16 Heller, M.
  2. Biochim. Biophys. Acta. v.1158 Abousalham, A.;Riviere, M.;Teissere, M.;Verger, R.
  3. Biochemistry v.18 Allgyler, T. T.;Wells, M. A.
  4. J. Biol. Chem. v.242 Yang, S. F.;Freer, S.;Benson, A. A.
  5. Biochemistry v.23 Bruzik, K.;Tsai, M. D.
  6. Methods in Enzymology v.72 Eibl, H.;Kovatchev, S.
  7. Biochem. Cell Biol. v.68 Ohno, Y.;Okazaki, M.;Hara, I.
  8. Korean Biochem. J. v.22 Lee, H.;Choi, M.;Koh, E.
  9. Biochemistry v.11 Chesebro, B.;Metzger, H.
  10. Anal. Chem. v.28 Chen. P. S.;Toribara, T. Y.;Warner, H.
  11. Biochim. Biophys. Acta. v.1084 Holbrook, P. G.;Pannell, L. K.;Daly, J. W.
  12. Bull. Korean Chem. Soc. v.13 Kim, C.;Koh, E.;Choi, M.

피인용 문헌

  1. Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata) vol.1437, pp.3, 1999, https://doi.org/10.1016/s1388-1981(99)00020-7