Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Insect Cell Culture for Recombinant $\beta$-galactosidase Production Using a Spin-filter Bioreactor

  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University) ;
  • Kim, Hak-Ryul (Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Ki-Woong (Department of Genetic Engineering, Kyung Hee University) ;
  • Kim, Tae-Yong (Department of Genetic Engineering, Kyung Hee University) ;
  • Oh, Jai-Hyn (Department of Genetic Engineering, Kyung Hee University) ;
  • Yang, Jai-Myung (Department of Biology, Sogang University)
  • Published : 1994.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Spodoptera frugiperda IPLB-SF-21-AE cells were cultivated in a spin-filter bioreactor with continuous perfusion for the recombinant $\beta$-galactosidase production. At the perfusion rate of 0.06 $hr^{-1}$, the maximum cell density of insect cells in this bioreactor system reached 3.5$\times$$l0^6$ viable cells/ml using the Grace media containing 5% FBS and 0.3% Pluronic F-68. The recombinant $\beta$-galactosidase production of 8, 100 units per reactor volume was also achieved at this perfusion rate.

Keywords