Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Volume Concentration Ratio of Cell-free Medium on Tetramethylpyrazine Production by Lactococcus lactis subsp. lactis biovar. diacetilactis FC1

  • Lee, Ji-Eun (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Woo, Gun-Jo (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University)
  • Published : 1994.09.01
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Abstract

Acetoin and ammonia, the precursors of tetramethylpyrazine (TMP) having "nutty " or "roasted" flavors, were produced by cultivating Lactococcus lactis ssp. lactis biovar. diacetilactis FC1. The effects of the volume concentration ratio (VCR) of cell-free medium on the formation of TMP were investigated using a rotary evaporator at $70^{\circ}C than at 80^{\circ}C$. As the VCR increased, the formation of TMP and the conversion ratio of acetoin to TMP increased. More TMP were formed at $70^{\circ}C than at 80^{\circ}C$. As the VCR increased, the concentration of acetoin decreased implying the formation of TMP from acetoin and ammonia.

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