Korean Journal of Plant Tissue Culture (식물조직배양학회지)
- Volume 21 Issue 3
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- Pages.173-176
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- 1994
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- 1015-5880(pISSN)
Effects of Medium Components on Discoloration an Necrosis of Cultures in Peony (Paeonia lactiflora Pall.) Micropropagation
작약의 미세번식에서 배지성분이 배양의 변색과 괴사에 미치는 영향
Abstract
Stem and petiol explants of peony culture turned to brownish black soon after placing onto medium and degenerated to death. Disroloration was caused mainly by ferrous and calcium cloride. Nitrate was a main factor for the death of culture. The culture damage was increased with the increment of the medium salt strength. A few latent axillary buds were elongated to shoots without forming callus.
작약절편을 접종하면 배지와 절편이 흑갈색으로 변하고 수 이로부터 배양이 죽기 시작하는데 배지성분 중에 이를 조장하는 물질이 있음을 발견하였다. 즉 변색의 주원인은 철분과 염화칼슘(Cacl