Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
권호 표지

백서의 초대 배양 간세포를 이용한 MTT assay$^{1)}$

MTT Assay Using Primary Cultured Rat Hepatocytes

  • 하헌 (효성여자대학교 약학대학) ;
  • 윤수홍 (효성여자대학교 약학대학) ;
  • 다케루 후지이 (제국제약) ;
  • 히토시 호리 (도쿠시마 대학)
  • Ha, Hun (College of Pharmacy, Hyosung Women's University) ;
  • Yoon, Soo-Hong (College of Pharmacy, Hyosung Women's University) ;
  • Fujii, Takeru (Teikoku Seiyaku) ;
  • Hori, Hitoshi (University of Tokushima)
  • 발행 : 1994.04.01
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초록

The tetrazolium dye, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), is reduced by live but not dead cell, and this reaction is used as the end point in a rapid drug screening assay. It can also be used for accurate determinations of drug sensitivity but only if a quantative relationship is established between cell number and MTT-formazan production. Several conditions were examined to devise an in vitro assay method in primary cultured hepatocytes, such as optimum wavelength, optimal MTT concentration, optimal incubation time, and cell density.

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