Regiospecificity of Reductive Dechlorination of Chlorophenols in Mono- and Di-Chlorophenol Adapted Anoxic Sediments

Mono-와 Di-Chlorophenol에 적응시킨 혐기성 저질의 탈염소 특성

  • 공인철 (미국 환경청 응용기술협회) ;
  • 이석모 (부산 수산대학교 환경공학과)
  • Published : 1994.03.01

Abstract

The regiospecific potential for the reductive dechlorination of 2-, 3-, 4-, 2, 3-, 2, 4-, and 3, 4-chlorophenols (CPs) was studied in mono- and di-CP(DCP) adapted sediment slurries(10% solids). Freshwater sediments adapted to transform 2-CP dechlorinated all tested mono- and di-CPs except 4-CP without a lag period. Adaptation to 2-CP, thus, enhanced the onset of dechlorination of 3-CP and all ortho-substituted CPs tested. Sediment adapted to transform 3-CP dechlorinated all test CPs, except 4-CP and 2, 4-DCP, without a lag period. Sediment adapted to individual DCPs (2, 3-, 2, 4-, and 3, 4-DCP_ exhibited dechlorination(no lag phase) of 2-CP, 2, 3-, 2, 4-, and 3, 4-CDP. Interestingly, meta-cleavage of 3, 4-DCP in all tested adapted sediment occurred, while para-cleavage occurred in 3, 4-DCP adapted sediment. Sediment adapted to dechlorinate ortho and meta-chlorines exhibited a preference for meta following ortho-cleavage, but not for para-cleavage, while the preference for reductive dechlorination was ortho>meta>para for mono-CPs and ortho>para>meta for DCPs in unadapted freshwater anoxic sediments.

자연호소의 혐기성 저질을 특정한 chlorophenol(CP)에 적응시킨 후 다른 구조물 가진 CP에 대한 탈염소 특성을 검토하였다. CP에 노출되지 않는 혐기성 저질에서는 mono-CP의 경우 ortho > meta > para-염소의 손으로 di-CP의 경우는 ortho > par > meta- 염소의 순서로 짧은 지체기를 거친 후 탈염소가 발생하였다. Mono-CP 중 2-CP에 적응된 저질은 4-CP와 3,4DCP를 제외하고, 3-CP에 적응시킨 저질은 4-CP를 제외한 모든 시험물질에 대하여 지체기 없이 탈염소 특성을 나타내었다. DCP에 적응된 모든 저질은 2-CP, 2,3,-, 2,4-, and 3,4-DCP를 지체기 없이 탈염소가 발생하지 않았다. 이 결과에서 볼 때 mono-와 di-CP를 탈염소시키는 혐기성 미생물의 종류가 다양함을 알 수 있다.

Keywords

References

  1. Appl. Environ. Microbiol. v.47 Anaerobic biodegradation of chlorophenol in fresh and acclimated sludge Boyd,S.A.;Shelton,D.R.
  2. Appl. Environ. Microbiol. v.45 Effects of metals on methanogenesis, sulfate reduction, carbon dioxide evolution and microbial biomass in anoxic salt Capone,D.G.;Reese,D.D.;Kiene,R.D.
  3. Wat. Res. v.23 Biodegradation of 2,4-dichlorophenol by activated sludge microorganisms Chudoba,J.;Albokova,J.;Lentge,B.;Kummel,R.
  4. Pentachlorophenol: chemistry, pharmacology, and environmental toxicology Patterns of pentachlorophenol usage in the United States of Americalan overview Circelli,D.P.;Rao,K.R.(ed.)
  5. Appl. Environ. Microbiol. v.55 Characterization of anaerobic dechlorinating consortia derived from aquatic sediments Genthner,B.R.S.;PriceII,W.A.;Pritchard,P.
  6. Microbial degradation of organic compounds Gibson,D.T.
  7. Microbiol. Ecol. v.20 Reductive dechlorination of dichlorophenols by nonadapted and adapted microbial communities in pond sediments Hale,D.D.;Rogers,J.E.;Wigel,J.
  8. Environ. Toxicol. Chem. v.10 Environmental factors correlated to dichlorophenol dechlorination in anoxic freshwater sediments Hale,D.D.;Rogers,J.E.;Wigel,J.
  9. Hurdey,S.E.;Knetting,E.;Daignault,S.A.;Fedorak,P.M.
  10. phenols. Environ. Technol. Lett. v.8 Anaerobic biodegradation of monochloro-
  11. Environ. Sci. Technol. v.13 Priority pollutants; a perspective view Keith,L.H.;Telliard,W.A.
  12. Appl. Environ. Microbiol. v.54 Dehalogenation in marine sediments containing natural sources of halophenols King,G.M.
  13. Appl. Environ. Microbiol. v.55 Anaerobic dechlorination of 2,4-dichlorophenol in freshwater sediments in the presence of sulfate Kohring,G.W.;Zhang,X.;Wiegel,J.
  14. Q195. Abstr. 92nd Gen. Meet. Am. Soc. Microbiol. Effects of heavy metals on the reductive dechlorination of chloropenols in anoxic freshwater sediment Kong,I.C.;Jones,W.J.
  15. Q377. Abstr. 93rd Gen. Meet. Am. Soc. Microbiol. Specificity of reductive dechlorination of chlorophenols in mono-and dichlorophenol adapted freshwater sediment Kong,I.C.;Jones,W.J.
  16. Water Res. v.22 Reductive dechlorination of chlorinated phenols in anaerobic upflow bioreactors Krumme,M.L.;Boyed,S.A.
  17. Appl. Environ. Microbiol. v.58 Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture Madsen,T.;Amand,J.
  18. Appl. Environ. Microbiol. v.58 Isolation and characterization of an anaerobic chlorophenol-transforming bacterium Madsen,T.;Light,D.
  19. Microbiol. Rev. v.56 Microbial reductive dehalogenation Mohn,W.W.;Tiedje,J.M.
  20. Microbial degradation of organic compounds Microbial degradation of halogenated aromatic compounds Reineke,W.;Gibson DT(ed.)
  21. Appl. Environ. Microbiol. v.48 Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3- chlorobenzoic acid Shelton,D.R.;Tiedje,J.M.
  22. Trends Biotechnol. v.3 Microbial egradation of chlorinated phenols Steiert,J.G.;Crawford,R.L.
  23. Science v.218 Dehalogenation : a novel pathway for the anaerobic biodegradation of haloaromatic compounds Sufita,J.M.;Horowitz,A.;Shelton,D.R.;Tiedje,J.M.
  24. Environ. Toxicol. Chem. v.4 Microbial metabolism of chlorophenolic compounds in ground water aquifers Suflita,J.M.;Miller,G.D.
  25. Microbial degradation of organic compounds Anaerobic degradation of aromatic compounds Young,L.Y.;Gibson DT(ed.)
  26. Appl. Environ. Microbiol. v.56 Sequential anaerobic degradation of 2,4-dichlorophenol in freshwater sediments Zhang,X.;Wigel,J.