Effect of ${\beta}-Carotene$ Supplementation on Lipid Metabolism and Related Enzyme Activities in Rats

${\beta}-Carotene$ 공급이 흰쥐 간 조직의 지질대사와 관련효소의 활성에 미치는 영향

  • 최은미 (영남대학교 식품영양학과) ;
  • 박정룡 (영남대학교 식품영양학과) ;
  • 서정숙 (영남대학교 식품영양학과)
  • Published : 1994.10.01

Abstract

This study was designed to investigate the effect of dietary ${\beta}-carotene$ level on the lipid metabolism and lipid peroxide metabolizing enzyme activities in rats. Male Sprague -Dawley rats were fed on diets containing five levels of ${\beta}-carotene$ (0, 10, 120, 1200, 12000mg/kg diet ; BC 0, BC 1, BC 2, BC 3, BC 4 group). The rats were sacrificed after 7 weeks of the feeding periods. Lipid peroxide value of mitochondrial fraction of rat liver was elevated in ${\beta}-carotene$ restriction group when compared to $\beta$ -carotene groups. Superxide dismutase activity increased significantly by ${\beta}-carotene$ supplementation. Both catalase and glutathione peroxidase activities were reduced with increasing ${\beta}-carotene$ supplementation, except only ${\beta}-carotene$ restriction group. In liver, the contents of total lipid and cholestero decreased by ${\beta}-carotene$ supplementation but triglyceride content was not different among treatment groups. HDL-and total cholesterol ratio in plasma of 12, 000 ${\beta}-carotene$ group decreased, and was similar to that of ${\beta}-carotene$ restriction group.

{\beta}-carotene$은 비타민 A 활성형인 retinol로 전환되어 그 기능을 수행하는 영양소로 알려져있지만 {\beta}-carotene$ 자체로서의 생리적, 영양학적 중요성이 부각되고 있다. 본 연구에서는 {\beta}-carotene$을 수준별로 공급하여 체내의 지질대사와 항산화 효소의 활성에 미치는 영향을 살펴보고자 계획되었으며 얻어진 결과는 다음과 같다. 간 미토콘드리아에서의 과산화지지르이 함량은 제한군과 과잉 공급군이 다른 처리군들에비해서 상대적인 증가를 보였고 BC 2군에서 가장 낮은 값을 나태내었다. SOD의 활성은 {\beta}-carotene$ 제한군이 공급군에 비해서 유의적으로 증가하여 SOD 활성에 대한 {\beta}-carotene$의 조절효과를 보여주었고 catalase와 GSH-Px의 활성에 대한 {\beta}-carotene$의 조절효과를 보여주었고 catalase와 GSH-Px의 활성은 과잉 공급군의 경우 가장 낮은 값을 보여주었다. {\beta}-carotene$ 공급에 따른 지질 함량의 변화는 간의 경우에 총지질 함량은 {\beta}-carotene$ 공급에 따라 감소하였고 중성지질은 전 군에서 유의적인 차이가 보이지 않았으며 인지질은 BC 2군과 BC 3군에서 유의적으로 낮은 값을 보였다. 콜레스테롤 함량은 BC 1군에서 가장 낮은 값을 보였다. 혈장에서는 {\beta}-carotene$ 공급이 증가함에 따라 중성지질의 함 은 증가하는 경향이었고 HDL-콜레스테롤은 BC 4군에서 가장 낮은 값을 나타내었다. HDL-콜레스테롤 함량비는 {\beta}-carotene$ 공급에 따라 증가되었으나 과잉공급군에서 제한군과 같은 수준으로 감소되었다. 이상의 결과에서와 같이 12,000mg의 {\beta}-carotene$을 급여한 군에서는 지질대사 관련효소 활성도와 체내 지질함량이 적정량을 급여한 군에 비해 크게 변화하였으나 10~1,200mg을 급여한 실험군들 사이에서는 큰 차이를 나타내지 않아 {\beta}-carotene$의 정량적 평가를 위한 지속적인 연구가 필요하다고 본다.하다고 본다.

Keywords

References

  1. J. Lipid Res. v.33 International conference on HDL-cholesterol and triglycerides:Role in coronary heart disease and laboratory measurement Brewer,H.B.;Leon,D.F.;Paoletti,R.;Sanghvi,A.
  2. J. Geron v.35 Aspects of free radical reactions in biological system Leibovitz,B.E.;Benjamin,V.S.
  3. Lab. Invest. v.47 Biology of disease:Free redicals and tissue injury Freeman,B.A.;James,D.C.
  4. Free Rad. Biol. Med. v.7 Lipid peroxidation:A mechanism for alcohol-induced testicular injury Rosenblum,E.R.;Gavaler,J.S.;Vanthiel,D.S.
  5. Nutr. Rev. v.37 Vitamin A, tumor initiation and tumor promotion Olson,J.A.
  6. J. Can. Clin. Oncol. v.116 Differential relationship vitamin A and E levels in methylnitrosourea-induced Sprageu-Dawley rats following prolonged feeding of fatty diets enriched with the vitamins Aksoy,M.;Berger,M.R.
  7. J. Nat. Cancer. Inst. v.79 Vitamin A,β-carotene and the risk of cancer:A prospective study Paganini,H.A.;Ann,C.;Ronald,K.S.;Brian,E.H.
  8. Am. J. Clin. Nutr. v.35 Distribution of α-tocopherol in human plasma lipoproteins Berens,W.A.;Thompson,J.N.;Madere,R.
  9. J. Biochem. v.219 Oxygen toxicity,oxygen redical,transition metals and disease Halliwell,B.;John,M.C.G.
  10. J. Nutr. v.107 Report of the American institute of nutrition Ad Hoc committe on standards for nutritional studies AIN Standard
  11. Met. Enz. v.55 Isolation of mitochondria with emphasis on heart mitochondria from small amounts of tissue Meta,L.;Seitz,S.
  12. Anal. Biochem. v.95 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Ohawa,H.;Ohishi,N.;Yagi,K.
  13. Methods of enzymatic analysis v.2 Catalase Aebi,H.;Bergmeyer,H.U.(ed.)
  14. J. Lab. Clin. Med. v.70 Studies on quantitative characterization of erythrocyte glutathione peroxidase Paglia,D.E.;Valentine,W.N.
  15. Biochem. Biophys. Res. Commun. v.71 Glutathione peroxidase activity in selenium-deficient rat liver Lawrence,R.A.;Burk,R.F.
  16. J. Lab. Clin. Med. v.85 The estimation of red cell superoxide dismutase activity Winterbourn,C.C.;Hawkins,R.E.;Brain,M.;Carrell,R.W.
  17. J. Biol. Chem v.226 A simple method for the isolation and purification of total lipids from animal tissues Folch,J.;Lees,M.;Stanley,G.H.S.
  18. Clin. Chem. v.21 Simple manual procedure for determination of serum triglycerides Mendez,J.B.;Gahagan,H.
  19. Anal. Biochem. v.29 Study of new cholesterol reagent Zlatkit,A.;Zak,B.
  20. Lipid v.3 The maturation for rat brain myelin Eng,L.F.;Noble,E.P.
  21. J. Biol. Chem. v.193 Protein measurement with the folinphenol reagent Lowry,O.H.;Rosebrough,N.J.;Fart,A.L.;Randall,R.J.
  22. J. Nutr. v.121 Comparative antioxidant effectiveness of dietary β-carotene, vitamin E, selenium and coenzyme $Q_10$ in rat erythrocytes and plasma Rosario,T.;Francisco,j.H.;All,T.
  23. J. Nutr. v.121 Canthaxanthin and excess vitamin A alter α-tocoperol, carotenoids and iron status in adult rats Blakely,S.R.;Mitchell,G.V.;Jenkins,M.Y.;Grundel,E.;Whittaker,P.
  24. Biochem. Biophys. Res. Commun. v.98 Free radical and singlet oxygen scavengers : Reaction of a peroxy-radical with β-carotene, diphenyl furan and 1,4-diazobicyclo(2,2,2)-octane Packer,J.E.;Mahood,J.S.;Mora-Arellano,V.O.;Slater,T.F.;Wolfenden,B.S.
  25. FEBS Letters v.238 Inhibition of adriamycin-promoted microsomal lipid peroxidation by β-carotene, α-tocopherol and retinol at high and low oxygen partial pressure Vile,G.F.;Christine,C.W.
  26. J. Nutr. v.119 β-Carotene and aryl hydrocarbon hydroxylase in the rat : An effect of β-carotene independent of vitamin A activity Edes,T.E.;William,T.;Jayendra,S.
  27. Fed. Proc. v.32 Lipid peroxidation damage to cell components Tapel,A.L.
  28. Toxicol. v.36 The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats Aykac,G.
  29. Am. J. Nutr. v.53 Lack of effect of chronic administration of oral β-carotene on serum cholesterol and triglyceride concentration David,W.N.;Garette,T.B.;There,A.S.
  30. N. Engl. J. Med. v.310 Relation of serum vitamin A and E and carotenoids to the risk of cancer Willett,W.G.;Pork,B.F.;Underwood,B.A.;Stampfer,M.J.;Pressel,S.;Rosner,B.;Taylor,J.O.;Schneider,K.;Hames,C.G.
  31. 한국영양학회지 v.24 비타민 A 과량 투여가 흰쥐의 체내 지방 대사상 및 장기의 비타민 A함량에 미치는 영향 김선효;이일하
  32. Biochem. Pharmac. v.30 Stimulation by adriamycin of rat heart and liver microsomal NADPH-dependent lipid peroxidation Mimnaugh,E.G.;Trush,M.A.;Gram,T.E.