Journal of Food Hygiene and Safety (한국식품위생안전성학회지)
- Volume 9 Issue 3
- /
- Pages.123-131
- /
- 1994
- /
- 1229-1153(pISSN)
- /
- 2465-9223(eISSN)
Competitive Enzyme-Linked Immunosorbent Assay for Detection of Gentamicin Residues in Edible Animal products
축산식품 중에 잔류하는 Gentamicin 검사를 위한 ELISA 개발에 관한 연구
- Kim, Jae-Myung (College of Veterinary Medicine, Seoul National University) ;
- Lee, Mun-Han (College of Veterinary Medicine, Seoul National University) ;
- Lee, Hang (College of Veterinary Medicine, Seoul National University) ;
- Ryu, Pan-Dong (College of Veterinary Medicine, Seoul National University) ;
-
Cho, Myung-Haing
(College of Veterinary Medicine, Seoul National University)
;
- Park, Jong-Myung (Veterinary Research Institute, RDA)
- 김재명 (서울대학교 수의과대학) ;
- 이문한 (서울대학교 수의과대학) ;
- 이항 (서울대학교 수의과대학) ;
- 류판동 (서울대학교 수의과대학) ;
-
조명행
(서울대학교 수의과대학)
;
- 박종명 (가축위생연구소)
- Published : 1994.09.01
Abstract
An enzyme-linked immunosorbent assay(ELISA) was developed for the detection of residual gentamicin(GM) in edible animal products. The immunogen(GM-KLH conjugate) and coating antigen(GM-BSA conjugate) were prepared by coupling GM sulfate to keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, respectively. Polyclonal antibody to GM was produced in rabbits(New Zealand White, female) by using the immunogen and the antibody titer was measured by indirect ELISA. A competitive ELISA was developed using GM-bovine serum albumin conjugate as a coating antigen, GM(as standards or sample), polyclonal antibody to GM, secondary antibody conjugated with horseradish peroxidase as an enzyme, and H2O2 and o-phenylenediamine dihydrochloride as a substrate and a chromophore, respectively. The detection limit of GM was 10 ng/ml and the standard curve of GM(n=26) was linear up to 10