The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 7 Issue 4
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- Pages.259-266
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- 1994
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Amylolytic Enzymes Produced from Hyperthermophilic Archaebactorium Thermococcus profundus
고도 호열성 Archaebacterium Thermococcus profundus가 생산하는 Amylolytic Enzymes
- Published : 1994.12.01
Abstract
The hyperthermophilic archaebacterium Thermococcus profundus Isolated from a deep-sea hydrothermal vent system, produced several amylolytic enzymes such as extracellular amylase and pullulanase, intracellular a-1,4-91ucosidase in respone to the presence of complex carbohydrates In the growth medium. This strain showed high activities on 0.5% maltose than on complex carbohydrates One of the amylases was partially purified by ammonium sulfate precipitation, DEAE-Toyopearl chromatography. The amylase exhibited maximal activity at pH 5.5 and 80