Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 9 Issue 2
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- Pages.161-166
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- 1994
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Production of cloned Rabbits by Nuclear Transplantation
핵이식에의한 복제토끼 생산
Abstract
This experiment was carried out to produce cloned aniraals by nuclear transplantation in rabbits. The ovulated oocytes were collected from the oviducts between 14 and 15 hours after hGG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The nucleated oocytes receiving a blastomere in the perivitelline space were electrically fused in the 0.28 M mannitol solution at 1.5 kV /cm, 60