한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권3호
  • /
  • Pages.296-303
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Trigonopsis variabilis의 고정화 및 Cephalosporin C로부터 7$\beta$-(4-Carbohybutanamido)Cephalosporanic Acid의 전환

Immobilization of Trigonopsis variabilis and Conversion of Cephalosporin C to 7$\beta$-(4-Caboxybutanamido)Cephalosporanic Acid

  • 김종균 (충북대학교 자연과학대학 미생물학과) ;
  • 임재윤 (충북대학교 자연과학대학 미생물학과)
  • 발행 : 1994.06.01
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초록

An immobilized Trigonopsis variabilis cells having an high activity of D-amino acid oxidase(DAO) was used to convert CPC into GL-7-ACA. The optimal pH of the reaction system was 8.0-8.5, and the optimal temperature was 40$\circ$C. When immobilized cell was used repeatedly in semi-batchwise reaction, the system retained 80% of the initial activity after used of 12 times for over 12 hours. The storage stability of the immobilized cell was maintained for 30 days at 4$\circ$C. The CPC concentration for the maximal reaction rate was about 30 mM and 40 mM for free and immobilized cells, respectively. Substrate inhibition of CPC concentration more than 50 mM was overcomed by 20~25% by immobilization. Pure oxygen supply into reaction system was most efficient in D-amino acid oxidase reaction. Continuous conversion to GL-7-ACA from CPC has been developed with an bioreactor system containing immobilized T variabilis cells. By opera- tion of the reactor for 5 hours, the average conversion yield of >80% and GL-7-ACA production of 40~45 mM per hour could be obtained.

키워드

참고문헌

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