Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Studies on the Protoplast Formation and Regeneration of Lactobacillus acidophilus 88

Lactobacillus acidophilus 88의 Protoplast 형성 및 재생에 관한 연구

  • Jun, Hong-Ki (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Heo, Kyeong (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Jo, Young-Bae (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Baik, Hyung-Suk (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 허경 (부산대학교 자연과학대학 미생물학과) ;
  • 조영배 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과)
  • Published : 1994.04.01
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Abstract

In the course of the study on strain inprovement by protoplast fusion, Lactobacillus acidophilus 88 protoplasts production and regeneration conditions were investigated. This strain produced a bacteriocin that revealed strong inhibitory activity against various indicator strains, especially L. helveticus CNRZ 1096. Protoplasts of L. acidophilus 88 strains were very efficiently obtained by treatment with 125 $\mu $g/ml lysozyme in a protoplast forming buffer containing 20 mM N-2 hydroxy-ethtl-piperazine-N'-2-ethane-sulfonic acid(HEPES, pH 7.0) and 1M sucrose at 37$\circ $C for 30 min. Hovever, treatment with mutanolysin was not effective for the production of L. acidophilus 88 protoplasts under the same conditions. High protoplast yield was obtained form the cells at the middle to late logarithmic growth phase in the de Man, Rogosa and Sharpe(MRS) medium. Regeneration was efficiently accomplished with the MRS medium containing 10% sucrose.

Keywords

References

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