Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of Replication origin from Enterococcal Plasmid p703/5

Enterococcus faecalis KBL 703 Plasmid p703/5의 Replication Origin의 Cloning

  • 전영욱 (고려대학교 자연자원대학 유전공학과) ;
  • 전세영 (고려대학교 자연자원대학 유전공학과) ;
  • 김영우 (고려대학교 자연자원대학 유전공학과) ;
  • 장효일 (고려대학교 자연자원대학 유전공학과)
  • Published : 1994.02.01
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Abstract

Enterococcus faecalis KBL703 has three plasmids(p703/9, p703/5 and p703/4). Within p703/5, the specific DNA region that would confer replication function(replication origin) was searched by transformation experiments. In order to use as the recipient of transformation, two plasmid-cured strainsd were made from this strain. Four recombinant DNA constructs, each containing fragment of p703/5 and CAT(chloramphenicol acetyl transferase) gene were also made. And they were used to transform the plasmid-cured strains. Only one DNA construct containing 3.6 kb SalI fragment was stably maintained as plasmid in these strains. Additional experiment using another Enterococcus faecalis strain(ATCC29212) as a recipient was successfully done and it was confirmed that this newly constructed recombinant plasmid plasimid contained the replication origin from p703/5 plamid.

Keywords

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