The Korean Journal of Zoology (한국동물학회지)
- Volume 37 Issue 4
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- Pages.488-494
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- 1994
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- 0440-2510(pISSN)
Purification and Characterization of Apolipophorin-III from Haemolvmph of Fall Webworm Hvphantria cunea Drury
미국흰불나방(Hyphantria cunea Drury) 혈림프부터 apolipophorin-III의 순수정제 및 특성
Abstract
Apolipophorin-III (ApoLp-III) was purified from adult haemolynph of Hyphantriu cuneo and their molecular weight and synthetic place were investigated. ApoLp-III purification was performed by KBr-density gradient ultracentrifugation followed by gel permeation chromatographv (Sephadex G-1001 and ion-exchange chromatography (CM-52) and their purity was confirmed on 10% SDS-PAGE. ApoLp-III has the molecular weight of 18 ItDa and is synthesized by fat body.