Archives of Pharmacal Research
- Volume 17 Issue 1
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- Pages.48-50
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- 1994
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
p-Coumaroylamino Acids from Yeast-Elicited Ephedra distachya Cultures
- Song, Kyung-Sik (Genetic Engineering Research Institute, KIST) ;
- Sankawa, Ushio (Faculty of Pharmaceutical Sciences, The University of Tokyo) ;
- Ebizuka, Yutaka (Faculty of Pharmaceutical Sciences, The University of Tokyo)
- Published : 1994.02.01
Abstract
Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.
Keywords
- Ephedra distachya;
- Ephedraceae;
- Cell suspension cultures;
- p-Coumaroyl-D-valine;
- p-coumaroyl-D-serine;
- p-Coumaroyl-D-threonine;
- Yeast elicitor