EFFECTS OF CARBONIC ANHYDRASE INHIBITORS ON THE LPS-INDUCED BONE RESORPTION IN VITRO

Carbonic Anhydrase Inhibitors가 Lipopolysaccharide에 의해 유도된 골흡수에 미치는 영향

  • Park, Yang-Ho (Department of Orthodontics, College of Dentistry, Dan Kook University) ;
  • Cha, Kyung-Suk (Department of Orthodontics, College of Dentistry, Dan Kook University)
  • 박양호 (단국대학교 치과대학 교정학교실) ;
  • 차경석 (단국대학교 치과대학 교정학교실)
  • Published : 1994.03.01

Abstract

To study bone resorption mechanism, effect of LPS on the $^{45}Ca$ release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied. Ulnae and radii were removed from 19 day old fetal rats, prelabelled by subcutaneous injection of $200{\mu}Ci\;^{45}CaCl_2$ into their mother on the 17th day of gestation. Radioactivities of $^{45}Ca$ released into media were determined after 24, 48 and 72 hours. Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of $\%$ release of $^{45}Ca$ between paired control and experimental group. The observed results were as follows : 1. $LPS(1{\mu}g/ml)$ supplemented in media for 72hours increased the $^{45}Ca$ release significantly after 48 and 72 hours of culture and $LPS(10{\mu}g/m1)$ increased the $^{45}Ca$ release significantly after 72 hours of culture. 2. LPS-induced $^{45}Ca$ release was not inhibited significantly by 1mM sulfanilamide but inhibited significantly by 10mM sulfanilamide after 48 and 72 hours of culture. 3. LPS-induced $^{45}Ca$ release was not inhibited significantly by 0.1mM dichlorphenamide but inhibited significantly by 1mM dichlorphenamide after 48 and 72 hours of culture. 4. LPS-induced $^{45}Ca$ release was not inhibited significantly by 1mM acetazolamide but inhibited sighificantly by 5mM acetazolamide after 72 hours of culture.

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