Korean Journal of Medicinal Crop Science (한국약용작물학회지)
- Volume 2 Issue 3
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- Pages.211-218
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- 1994
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- 1225-9306(pISSN)
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- 2288-0186(eISSN)
Establishment of in Vitro Culture and Effect of Chilling Treatment of Mother Bulb On Bulblet Formation in Fritillaria thunbergii Miq.
패모의 초기배양과 모구의 저온처리가 자구형성에 미치는 영향
- Yu, Kwang-Jin (Department of Horticulture, Chungbuk National University) ;
- Paek, Kee-Yeoup (Department of Horticulture, Chungbuk National University) ;
- Seong, Nak-Sul (Medicinal Crop Division, Crop Experiment Station, RDA) ;
- Choi, In-Sick (Chungbuk provincial RDA) ;
- Cho, Jin-Tae (Chungbuk provincial RDA)
- Published : 1994.09.30
Abstract
This experiment was conducted to obtain basic information for the establishment of in vitro initial culture system in Fritillaria thuubergii Miq. Methods of surface sterilization of scale segments as explant and effect of antibiotics added into the culture medium on contamination of explant and chilling treatment of mother bulb on bulblet formation were investigated. Portent of contamination of cultured scale segments was significantly higher in the outer scale segments which were unsuitable as initial culture explant than inner scale segments. Contamination of explants taken from inner scale of bulb was reduced by surface sterilizing explants in the solution of
조직배양에 의한 패모(Fritillaria thuubergii Miq)의 대량번식 체계를 확립하기 위한 기초실험으로써 인편배양시 오염을 감소에 미치는 모구의 표면살균방법, 항생제처리 효과, 모구의 조온처리 기간이 자구형성에 미치는 영향을 구명하고자 실험한 결과는 다음과 같다. 1. 포장재배한 패모를 수확한 후 인편 배양하였을 경우 외측인편이 내측인편보다 오염율이 현저히 높아 배양재료로는 부적합하였고, 내측인편은 70% 에탄을에 30분간 침지후
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