Antioxidant Components of Gardenia Fruit

치자의 항산화 활성성분에 관한 연구

  • Han, Yong-Nam (Natural Products Research Institute, Seoul National University) ;
  • Oh, Hee-Kyung (Department of Foods & Nutrition, Duksung Women's University) ;
  • Hwang, Keum-Hee (Department of Foods & Nutrition, Duksung Women's University) ;
  • Lee, Mie-Soon (Department of Foods & Nutrition, Duksung Women's University)
  • 한용남 (서울대학교 천연물과학연구소) ;
  • 오희경 (덕성여자대학교 식품영양학과) ;
  • 황금희 (덕성여자대학교 식품영양학과) ;
  • 이미순 (덕성여자대학교 식품영양학과)
  • Published : 1994.09.30

Abstract

An antioxidant activity of Gardenia Fruit (Gardenia jasminoides Ellis) which has been used for food coloring was studied. The antioxidant activity was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). Both water and methanol extracts of Gardenia Fruit showed the antioxidant activity. On solvent fractionation, the antioxidant activity was removed into the ethyl acetate and butanol soluble fractions. And the final water soluble fraction also showed the antioxidant activity in the low concentration, but it promoted the lipid peroxidation in the high concentration. Two compounds (I and II) having the antioxidant activity were isolated from the butanol fraction, and compound I also occurred in the ethyl acetate fraction. The antioxidant activity of compound II was more potent than that of I. By analyzing data for UV, IR and $^1H-NMR$, compounds I and II were identified as geniposide and crocin, respectively.

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