Clinical and Experimental Reproductive Medicine
- Volume 20 Issue 2
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- Pages.165-176
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- 1993
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Studies on Quality Control by Frozen-Thaw 2-Cell Mouse Embryos
냉동보존된 생쥐배아를 이용한 정도관리에 관한 연구
- Han, Sun-Nam (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Kim, Hyang-Mee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Jung, Hae-Won (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Oh, Seung-Eun (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Son, Young-Soo (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Yu, Han-Ki (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Ahn, Jung-Ja (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
- Woo, Bock-Hee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University)
- 한선남 (이화여자대학교 의과대학 산부인과학교실) ;
- 김향미 (이화여자대학교 의과대학 산부인과학교실) ;
- 정혜원 (이화여자대학교 의과대학 산부인과학교실) ;
- 오승은 (이화여자대학교 의과대학 산부인과학교실) ;
- 손영수 (이화여자대학교 의과대학 산부인과학교실) ;
- 유한기 (이화여자대학교 의과대학 산부인과학교실) ;
- 안정자 (이화여자대학교 의과대학 산부인과학교실) ;
- 우복희 (이화여자대학교 의과대학 산부인과학교실)
- Published : 1993.08.31
Abstract
These studies were carried out to investigate the optimal freezing protocol for 2 cell mouse embryos and to find the probability of quality control with 2-cell embryos frozen. The embryos showed the best survival by the protocol composed of a freezing solution with the cryoprotectants(1.5M propanediol + 0.1M sucrose), and a 2-steop thawing method(room temperature, 20 sec-37
Keywords