Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Active metabolites in rat bile after intravenous injection of [3H] pteroylglutamic acid

랫드에 있어서 [3H] pteroylglutamic acid 의 정맥주사후(靜脈注射後) 담즙중(膽汁中) 활성대사물(活性代謝物)에 관한 연구(硏究)

  • Shin, Ho-chul (Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology) ;
  • Shimoda, Minoru (Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology) ;
  • Kokue, Eiichi (Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology)
  • Received : 1993.09.20
  • Published : 1993.10.30
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Abstract

Active metabolites in rat bile after an intravenous injection of $[^3H]$ pteroylglutamic acid(PteGlu)were studies using high-performance liquid chromatography(HPLC). Predominant four radioactive metabolites and parent compound PteGlu were detected on the chromatogram of HPLC with liquid scintillation counting system. These metabolites were identified as tetrahydrofolate, 10-formyltetrahydrofolate, 5-methyltetrahydrofolate and para-aminobenzoyl glutamate. The identification of active folate metabolites was based on the consistency of retention time profiles and hydrodynamic voltammograms which were obtained by HPLC with the electrochemical detection system, and characteristics of UV absorbance spectra obtained by HPLC with photodiode array detection system.

항암치료의 조절인자로서 중요시되는 활성엽산대사물의 장간(腸肝)순환동태를 규명하기 위해 랫드에 $[^3H]pteroylglutamic$ acid(PteGlu)를 정맥주사한 후 고성능 액체 크로마토그래피법(HPLC)을 이용해 담즙중 활성대사물에 관해 조사하였다. HPLC-liquid scintillation counting법의 분석결과, 현저한 다섯개의 방사활성 피크가 출현, 이들은 $H_4PteGlu$, $10-HCO-H_4PteGlu$, $5-CH_3-H_4PteGlu$와 paraaminobenzoyl glutamate(pABG), PteGlu로 각각 동정되었다. 전자 3물질의 활성엽산대사물의 동정은 표준물질과의 비교분석에 의해 HPLC-전기화학검출기를 이용한 retention time profile 및 hydrodynamic, Voltammogram의 일치성 그리고 HPLC-photodiode array분석에 의한 흡광스펙트럼의 일치성에 근거하였다. 후자 그 물질의(pABG 및 PteGlu) 동정은 흡광스펙트럼의 일치성에 근거하였다. 랫드 담즙중$[^3H]PteGlu$의 활성엽산대사물로서 $H_4PteGlu$의 존재가 처음으로 밝혀졌다.

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