대한수의학회지 (Korean Journal of Veterinary Research)
- 제33권2호
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- Pages.337-343
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- 1993
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
개에서 Hamster test의 이용을 높이기 위한 정액처리조건
Semen treatment to enhance the use of hamster test in the dog
- Kim, Yong-jun (College of Veterinary Medicine, Chonbuk National Universtiy) ;
- Lee, Hae-iee (College of Veterinary Medicine, Chonbuk National Universtiy)
- 투고 : 1993.03.29
- 발행 : 1993.04.30
초록
To determine the test conditions to enhance the use of hamster test in dogs, semen were collected from four dogs which had been proven to be fertile in the past and then preserved in BWW (Biggers, Whitten, Whittingham) medium for about 20 hours. The semen were given each different treatment according to the experimental design and coincubated with zona-free hamster ova for 5 hours. The ova were stained by lacmoid and examined under phase contrast microscope to investigate the rates of ova bound with sperm(sperm binding) and ova penetrated by sperm (penetration), and also numbers of both bound and penetrated sperm per ovum. In comparison of different concentrations of canine sperm, the rate of sperm binding was higher in
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