Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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STABLE TRANSFORMATION OF CULTURED CHICKEN CELLS

  • Han, J.Y. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Shin, Y.S. (Department of Animal Science, Shingu Junior College) ;
  • Shoffner, R.N. (Department of Animal Science, University of Minnesota) ;
  • Guise, K.S. (Department of Animal Science, University of Minnesota)
  • Received : 1993.05.31
  • Accepted : 1993.08.13
  • Published : 1993.12.01
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Abstract

A plasmid vector, $RSVLTR/{\beta}G2$, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid $RSVLTR/{\beta}G2$ was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of $RSVLTR/{\beta}G2$. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.

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