Control of Jujube Witches'-Broom by Post-Harvest Trunk Injection of Oxytetracycline

옥시테트라싸이클린의 수확후수간주입(收穫後樹幹注入)에 의(依)한 대추나무 빗자루병(病) 방제(防除)

Post-harvest trunk injection of oxytetracycline-HCl(OTC) was attempted for the control of jujube witches'-broom. Diseased jujube(Zizyphus jujuba) trees with 9 to 16cm trunk diameters were treated with OTC ranged from 2g to 6g according to the size of trunk diameter. OTC dissolved in 0.51 or 1.01 of water was transfused into diseased trees by gravity flow injection during September and October. All these OTC concentrations prevented symptom development for two growing seasons and restored previously severely diseased trees to normal conditions. There was no differences in control effect between 0.51 and 1.01 OTC solutions. With these high OTC concentrations, no phytotoxicity was observed in the new leaves of the following year. Mycoplasma -like organism (MLO)-specific fluorescence was absent in the phloem of recovered tissue when examined by fluorescence microscopy using DAPI(4'-6-diamidino-2-phenylindole.2HCl) staining, indicating the disappearance of MLO by the action of OTC.

대추를 수확(收穫)한 직후(直後)에 oxytetracycline-HCl(OTC)용액(溶液)를 수간주입(樹幹注入)하여 대추나무 빗자루병(病)에 대(對)한 방제효과(防除效果)를 조사(調査)하였다. 빗자루병(病)에 결린 대추나무(흉고직경(胸高直徑) 9-16cm)에 크기에 따라 약량(藥量)을 늘리면서 주당(株當) OTC 2g 내지 6g을 물 0.51 및 1.01에 녹여 9월(月)에서 10월(月) 사이에 중력식(重力式) 수간주입(樹幹注入)을 실시(實施)한 결과(結果), 2년간(年間) 병징(病徵)이 억제(抑制)되고 건전(健全)한 모습으로 회복(回復)되었다. 0.51와 1.01수용액간(水溶液間)에 방제효과(防除效果)의 차이(差異)가 없었으며, 이와 같이 비교적(比較的) 고농도(高濃度)의 OTC를 주입(注入)하였을 경우(境遇) 우려(憂慮)되는 약해(藥害)가 이듬해 새로 나온 잎에서 관찰(觀察)되지 않았다. 한편 회복(回復)된 나무의 사부조직(篩部組織)을 DAPI(4'-6-diamidino-2-phenylindole.2HCl)로 염색(染色)하여 형광현미경(螢光顯微鏡)으로 검경(檢鏡)하여 보았던 바 마이코플라스마-특이(特異) 형광반응(螢光反應)이 나타나지 않음으로써 조직내(組織內)의 마이코플라스마가 소멸(消滅)되었음을 알 수 있었다.