한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제21권4호
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- Pages.329-335
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- 1993
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
오이조직 배양세포에 의한 Ascorbate Oxidase 생성 및 생산
Formation and Production of Ascorbate Oxidase by Cucumber Tissue Cultured Cells
- Lee, Jong-Hwa (Department of Microbial Technology, Kon-kuk University) ;
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Chung, Ho-Kwon
(Department of Microbial Technology, Kon-kuk University) ;
- Shinmyo, Atsuhiko (Department of Biotechnology, Osaka University) ;
- Lim, Bun-Sam (Research and Development Center Miwon Co., LTD.)
- 발행 : 1993.08.01
초록
Ascorbate oxidase activity in various cucumber tissue extracts was highest in young fruit peeling. Cucumber callus was induced from young fruit peeling and callus cell lines were selected for more than 7 months, which porduced high levels of ascorbate oxidase and had a high growth rate. Induction of callus was optimized with Linsmaier-Skoog(LS) medium at 25