Effects of Polyamines on Chlorophyll and Protein content, and $\delta$-Aminolevulinate Dehydratase Activity in Greening Mung Bean Cotyledons

녹두자엽에서 엽록소 및 단백질함량과 $\delta$-Aminolevulinate Dehydratase활성에 미치는 Polyamine의 영향

Effects of polyamines on chlorophyll and protein content, and $\delta$-aminolevulinate dehydratase(ALAD) activity were investigated during the greening of mung bean cotyledons. Polyamines stimulated chlorophyll formation in greening cotyledons, and this effect was enhanced by KCl. The changes in protein content were similar to the changes for chlorophyll content. The excision entailed an increase in ALAD activity. Then a decrease appeared after 48 h incubation on water in the dark. It was more precocious in the light, but was accelerated when the cotyledons were illuminated after a dark preincubation. Putrescine had little effect on ALAD activity in the dark. In the light, putrescine prevented the decrease in ALAD activity and enhanced this activity when a dark preincubation preceded illumination. KCl had a slight stimulating effect in the dark, but was uneffective in the light. The combination putrescine＋KCl was devoid of stimulating effect. The results obtained suggest that plastid development of mung bean cotyledons during greening was affected by polyamines and light and that polyamines may play a role in the regulation of plastid development.

녹두 유식물의 자엽에서 엽록소 및 단백질 함량과 $\delta$-aminolevulinate dehydratase(ALAD)활성의 변화에 미치는 polyamine의 영향을 조사하였다. Polyamine은 녹화과정에서 자엽내의 엽록소 생성을 촉진하였으며, 이 효과는 KCl에 의해서 상승되었다. 자엽내의 단백질 함량의 변화 또한 엽록소 함량의 변화와 유사하였다. ALAD활성은 암하에서보다 광선하에서 억제되었으나, 18시간 암처리후의 광조사는 ALAD활성을 증가시켰다. Putre-scine처리에 의한 ALAD활성은 암하에서 촉진효과가 낮았으나 광선하에는 그 활성이 증가되었다. KCl은 암하에서 ALAD활성을 촉진시켰으나 광선하에서는 그 효과가 감소되었다. 또한 polyamine과 KCl의 복합처리에서는 촉진효과가 없었다. 이와같은 결과에서 녹두자엽에서의 색소체발달은 polyamine과 광선에 의해 영향을 받으며, polya- mine은 색소체발달에 중요한 작용을 하는 것으로 사료된다.