Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)
- Volume 22 Issue 3
- /
- Pages.340-348
- /
- 1993
- /
- 1226-3311(pISSN)
- /
- 2288-5978(eISSN)
Cross-Synergistic Interactions between Trichoderma viride and Penicillium funiculosum Cellulase
Trichoderma viride와 Penicillium funiculosum Cellulase 성분효소 간의 상승작용에 관한 연구
Abstract
Cross-synergistic interactions were evaluated with purified enzymes from Trichoderma viride and Penicillium funiculosum cellulase. Different synergistic patterns between enzyme components were observed. Exo-exo type synergism was found to be the most effective for degrading Avicel in all cases. Exo-endo type synergism was found to be slightly less effective. Extended hydrolysis of Avicel was carried out using mixtures of purified enzyme components with the crude cellulase from a different source. Addition of
Penicillium funiculosum과 Trichoderma viride cellulase의 정제효소들을 사용하여 cross-synergism을 조사하였다. Exo-exo형 상승작용이 Avicel을 분해하는데 가장 효과적이었으며 exo-endo형은 다소 효과가 떨어졌다. 정제효소성분과 이종의 조효소를 혼합하여 70시간 이상 Avicel을 가수분해하며 생성된 total sugar를 측정한 결과, P. funiculosum cellulase에서 분리정제한