Changes in the Concentration of Tocopherol and Carotenoid in Pinenut Oil during Autoxidation

잣 지방질의 자동산화에 따른 토코페롤 및 카로티노이드의 변화

The changes in the concentration of toropherol and carotenoid in pinenut oil at various stage of refining are studied during autoxidation. The oxidative stability of pinenut oil decreased with the refining degree increased. The tocopheol content of total crude oil was 55.51mg/100g oil and $\alpha$, ${\gamma}$and $\beta$-tocopherol in the crude oil were 25.48mg, 23.94mg and 6.99mg in order, respectively. The amount of $\delta$-tocopherol was trace. The concentrations of $\alpha$, ${\gamma}$ and $\beta$-tocopherol in pinenut oil after degumming or alkaline refining were 23. 85mg, 19.79mg and 6.12mg or 24.08mg, 24.04mg and 5.33mg, respectively. The content of f-carotene was 0.63% of total unsaponifiable materials and that of lycopene was trace. The concentrations of carotenoids and toropherols in pinenut oil decreased while autoxidation progressed. Degrees of destruction of carotenoids and toropherols. were significant at first stage of oxidation. Decrease in $\alpha$-toropherol was found to be faster than that in $\alpha$, ${\gamma}$-tocopherol during oxidation.

잣 지방질을 정제 단계를 달리하여 자동산화 시키면서 토코페롤 및 카로티노이드의 변화를 살펴 보았다. 정제 단계를 거칠수록 산화 안정성이 감소되었으며 총 토코페롤의 함량은 조지방의 경우 55.51mg/100g oil이었으며 이 중 $\alpha$-토코페를 25.48mg, ${\gamma}$-토코페를 23.94mg, $\beta$-토코페를 6.99mg순으로 함유되어 있었으며 $\delta$-토코페롤은 흔적량 존재하였다. 탈검된 지방질 및 탄산된 지방질에서도 $\alpha$-토코페를이 23.85mg, 24.08mg, ${\gamma}$-토코페롤이 19.79mg, 20.04mg, 그리고 $\beta$-토코페롤은 6.12mg, 5.33mg함유되어 있었다. 카로티노이드 중 $\beta$-카로틴은 비 비누화물질 중의 0.63%이었으며 리코펜은 미량 함유되어 있었다. 지방질의 자동산화가 진행되는 동안 카로티노이드와 토코페롤은 감소되었으며, 특히 자동산화의 초기과정에서 항산화제로 작용하여 상당량이 감소된 것으로 여겨졌으며 그 감소속도는 $\alpha$-토코페롤, ${\gamma}$-토코페롤 순이었다.