Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Studies on the Primary Structure of the Alkaline Protease in Neungee [Sarcodon aspratus (Berk.) S. Ito] I. Amino Acid Composition, Chemical Modification and Sequence of the N-terminal Amino Acid

능이[Sarcodon aspratus(Berk.) S. Ito]중 알카리성 단백질가수분해효소의 1차구조에 관한 연구 I. 아미노산 조성, 활성부위 아미노산 및 N-말단 부위의 아미노산 배열

  • 이태규 (전주우석대학교 식품공학과)
  • Published : 1993.12.01
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Abstract

Properties of a protease purified from Sarcodon asparatus(Berk.) S. Ito have been investigated. The enzyme displays as a glycosylated serine protease. The sequence for the 21 amino acids of the N-terminal side in the enzyme was determined by automated sequence analysis. The sequence was V-T-T-K-Q-T-N-A-P-W-G-L-G-N-I-S-T-T-N-K-L-.

Sarcodon aspratus(Berk.) S. Ito에서 분리정제한 단백질가수분해효소의 특성을 조사하였다. 이 효소는 당을 2.1% 함유하고 있었다. Rose bengal, N-bromo succinimide, phenyl methyl sulfonyl fluoride(PMSF)와 같은 화학적 수식 시약에 효소 활성이 저해되었으며, 1차 반응속도론적 불활성 mode를 가지므로 활성 부위는 tryptophan과 serine으로 추정된다. N-말단에서 21번째 잔기까지의 아미노산 배열은 V-T-T-K-Q-T-N-A-P-W-G-L-G-N-I-S-T-T-N-K-L-으로 동정되었다.

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References

  1. Food biotechnology Knorr,D.
  2. Source book of food enzymology Schwimmer,S.
  3. J. Kor. Soc. Food Nutr. v.17 no.1 Purification and some charcteristics of the proteolytic enzyme in fruitbody of Neungee Lee,T.K.
  4. J. Kor. Pharm. Sci. v.19 no.2 Purification and stability of proteolytic enzyme in Sarcodon aspratus(Berk.) S. Ito. Lee,T.K.;Eun,J.S.;Yang,J.H.;Jo,D.Y.;Yang,H.C.
  5. Chromatofocusing with Polybuffer™ and PBE™ manual : Pharmacia fine chemicals(Revised edition)
  6. Biochem. v.60 Halogenation of tyrosine and hydrolysis Sanger,F.;Thompson,E.O.D.
  7. Anal. Biochem. v.60 A new acid hydrolysis method for determining tryptohan in peptides and proteins Penke,B.;Ferenczi,R.;Kovacs,K.
  8. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois,M.;Gilles,K.A.;Hamilton,J.K.;Robers,P.A.;Smith,F.
  9. Chemical modification of proteins Means,G.E.;Feeney,R.E.
  10. J. Virology v.12 Processing of adenovirus 2-induced proteins Anderson,C.W.;Baum,P.R.;Gestelnad,R.F.
  11. The enzymes v.Ⅲ Boyer,P.D.
  12. J. Biol. Chem. v.26 Biosynthesis of Trypanosama brucei varient surface glycoproteins Ferguson,M.A.J.;Duszeks,M.;Lamont,G.S.;Overath,P.;Gross,G.A.M.