The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 6 Issue 3
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- Pages.199-207
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- 1993
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Isolation and Identification of Trichoderma sp. HK 47 Producing Avicelase
Avicelase 생산성 Trichoderma sp. HK 47의 분리 및 동정
Abstract
In order to obtain a good microorganism capable of degrading microcrystaline cellulose (avicel), the screening test was carried out from soil and brown-rot wood. 8 strains which had good avicel-hydrolyzing activity were isolated. Among them, HK 47 which exhibited the highest avicel hydrolyzing activity was identified as Trichoderma sp. HK 47. Maximum avicel-hydrolyzing enzyme production from Trichoderma sp. HK 47 was obtained with the optimum medium contained carboxymethylcellulose 1.5% as carbon source, NaN030.75% as nitrogen source, KH2P040.5%, MgSO4.7H2O 0.1%, Tween 800.005% (V/V) during stationary cultivation at pH 6.0, 3