한국수정란이식학회지 (Journal of Embryo Transfer)
- 제8권1호
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- Pages.31-36
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- 1993
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Ethylene Glycol을 이용한 유리화 동결시 배 발달단계별 생쥐배의 생존성
Post-thaw Survival of Mouse Embryos of Various Developmental Stages Cryopreserved by Vitrification in Ethylene Glycol-Based Solution
초록
The post-thaw survival of mouse embryos of the various developmental stages was determined after cryopreservation by vitrification in a solution containing ethylene glycol, Ficoll and sucrose (EFS). All the embryos were equilibrated for 2 minutes just prior to freezing. The number of blastomeres during in vitro development was counted by nuclei higher rates of post-thaw survival were obtained from the embryos of 2-cell(92.2%), 8-cell(77.2%) or morula stage(90.0%) than those of blastocyst stage(62.7%). The number of blastomeres per embryo following in vitro culture for 24 hours was significantly(P<0.05) smaller as 66.0f22.3 in vitrified and thawed morulae than fresh morulae(91.7
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