Bacillus sp. LK-1의 Exopolysaccharides(POL-II) 생산 및 특성

The Production and Properties of Exopolysaccharides(P0L-11) by Bacillus sp. LK-1

  • 김양효 (한솔제지) ;
  • 안성구 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 서현호 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 김혜자 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소 미생물공학연구실)
  • 발행 : 1993.10.01

초록

The strain which produced highly viscous exopolysaccharides (EPS) in liquid culture was selected from soil. The strain was supposed to Bacillus sp. from the results of mophological, biochemical and physiological tests. The medium composition for EPS production was trypton 0.75%, sucrose 4%, CaCO3 0.01%, Winogradsky's nitrogen free mineral medium 5ml/l and pH 7.0. In 2-l jar fernenter, the viscosity of culture broth after 120-hr cultivation time was very high (60, 000 cps) and the amount of EPS was 6.2g/l.

키워드

참고문헌

  1. Agri. Biol. Chem. v.53 Reological properties of gellan gum in aqucous media Tako,M.;A.Sakae;S.Nakamura
  2. Agri. Biol. Chem. v.53 Electron microscopy of the gel-forming ability of polysaccharide food additives Kanazawa,Y.;A.Koreeda;A.Harada;T.Harada
  3. Progress in industrial microbiology, Vol. 18, Some novel bacterial polysaccharides of recent development Kang,K.S.;G.T.Veeder;I.W.Cottrell;M.E.Bushell(eds.)
  4. Appl. Environ. Microbiol. v.37 Emulsifier of Athrobacter RAG-1. Isolation and emusifying properties Rosenberg,E.;A.Zuckerberg;C.Rubinovitz;D.L.Gutnick
  5. Appl. Microbiol. Biotechnol. v.32 Emulsifying agents from bacteria isolated during screening for cells with hydrophobic surfaces Neu,T.R.;K.Poralla
  6. Biotechnol. Bioeng. v.20 Mixing, mass transfer, and scale-up of polysacharide fermentation Margaritis,A.;J.E.Jajic
  7. ACS Sym. Ser. v.41 Production of industrially impotant gum with particular reference to xanthan gum and microbial alginate Lawson,C.J.
  8. ACS Sym. Ser. v.126 Industrial potential of fungi and bacterial polysaccharides Cottrel,I.W.
  9. Adv. Appl. Microbial. v.23 Production of microbial polysaccharides Slodki,M.E.;M.C.Cadmus
  10. Comprehensive Biotechnol v.3 Microbial polysaccharides Margaritis,A.;G.W.Pace;M.Moo-Yoong(ed.)
  11. SGM Symp. Ser. v.29 In Microbiol Technology Sutherland,I.W.;D.C.Ellwood;Bull,A.T.(ed.);D.C.Ellwood(ed.);C.Ratledge(ed.)
  12. Food hydrocolloides v.1 Glicksman,M.
  13. Bergy's manual of systimatic bacteriology(2nd eds.) v.2 Peter,H.A.S.;S.M.Nicholas;M.E.Sharpe;J.G.Holt
  14. Biochemical tests for identification of medical bacteria(2nd edition) Macfaddin,J.F.
  15. Methods in Microbiology v.19 Komagata,K.;K.Suzuki;R.R.Colwell(ed.);R.Grigorova(ed.)
  16. Bergey's manual of Systimatic bacteriology v.1 Tchan,Y.T.;P.B.New;N.R.Krieg(ed.);J.G.Holt(ed.)
  17. Microbial polysaccharides and polysaccharides Pullullan synthesis by Aurobasidium pullulans Catly,B.J.;R.C.W.Berkeley(eds.)
  18. Adv. Sympo. Ser. v.45 Extracellular polysaccharides Sutherland,I.W.
  19. Appl. Environ. Microbiol. v.44 Exopolysaccharide distribution and bioemulsifier prodution by Acinetobacter calcoaceticus BD4 and BD413 Kaplan,N.;Z.Rosenberg
  20. J. Gen. Microbiol. v.107 Investigation of the effect of environmental condition on the rate of exopolysaccharides synthesis in Azotobacter vinelandii Jarman,T.R.;L.Deavin;S.Slocombe;R.C.Righelato
  21. Polysacharides in Microbial Technology(2nd.) v.1 Kang,K.S.;I.W.Cottrel;H.J.Peppier(ed.);D.Periman(ed.)