Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hormonal Regulation of Glycerol-Phosphate Acyltransferase Gene Expression

Glycerol-Phosphate Acyltransferase Gene Expression의 호르몬에 의한 조절

  • 손승렬 (단국대학교 미생물학과) ;
  • 신동훈 (고려대학교 식량공학과)
  • Published : 1993.10.01
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Abstract

Both glycerol-phosphate acyltransferase (GPAT) and 7.2 kb mRNAs were present at the highest level in liver. Glycerol-phosphate acyltransferase and 7.2 kb mRNA levels increased dramatically when fasted mice were refed a high carbohydrate diet. In mature 3T3-L1 adipocytes, insulin increased both glycerol-phosphate acyltransferase and 7.2kb mRNA levels 2.6 to 3-fold while dibutyryl cAMP decreased mRNA levels by 50% and 80%, respectively. These results indicate positive regulation by insulin and negative regulation by dibutyryl cAMP of both glycerol-phosphate acyltransferase and 7.2 kb mRNA.

Keywords

References

  1. Biochemistry of Lipids and Membranes Brindly,D.N.;D.E.Vance(ed.);J.E.Vance(ed.)
  2. The Enzymes Bell,R.M.;R.A.Coleman;P.D.Boyer(ed.)
  3. J. Biol. Chem. v.251 Triacylglycerol synthesis in isolated fat cells Schlossman,D.M.;R.M.Bell
  4. J. Biol. Chem. v.255 Selective changes in enzymes of the sn-glycerol 3-phosphate and dihydroxyacetone phosphate pathwats of triacylglycerol biosynthesis during differentiation of 3T3-L1 preadipocytes Coleman,R.A.;R.M.Bell
  5. J. Biol. Chem. v.247 A substrate- and position-specific acylation of sn-glycerol 3-phosphate acyltransferase by rat liver mitochondria Monroy,G.;F.H.Rola;M.E.Pullman
  6. Mol. Cell. Biochem. v.12 The glycerophosphate acyltransferases and their function in the metabolism of fatty acids Bremer,J.K.;S.Bjerve;B.Borrebaek;R.Christiansen
  7. Biolchem. J. v.182 A study of the glycerol phosphate acyltransferae and dihydro-xyacetone phosphate acyltransferase activities in rat liver mitochondrial and microsomal fractions Bates,E.J.;E.D.Saggerson
  8. Biochem. J. v.243 Effects of streptozotocin-diabetes and insulin administration in vivo or in vitro on the activities of five enzymes in the adipose-tissue triacylglycerol synthesis pathway Saggerson,E.D.;C.A.Carpenter
  9. J. Biol. Chem. v.258 The DNA sequences encoding plsB and dgk loci of Escherichia coli Lightner,V.A.;R.M.Bell;P.Modrich
  10. J. Biol. Chem. v.258 Partial NH₂and COOH-terminal sequence and cyanogen bromide peptide analysis of Escherichia coli sn-glycerol 3-phosphate acyltransferase Green,P.R.;T.C.Vanaman;P.Modrich;R.M.Bell
  11. J. Biol. Chem. v.264 Hornal regulation of mouse fatty acid synthase gene transcription in liver Paulauskis,J.D.;H.S.Sul
  12. J. Biol. Chem. v.263 Cloning and expression of mouse fatty acid synthase and other specific mRNAs Paulauskis,J.D.;H.S.Sul
  13. Biochemistry v.19 Changes in the hepatic levels of messenger ribonueleic acid for malic enzyme during induction by thyroid hormone or diet Towle,H.C.;C.N.Mariash;J.H.Oppenheimer
  14. Proc. Natl. Acad. Sci. v.77 Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose Thomas,P.S.
  15. Anal. Biochem. v.132 A technique for radiolabeling DNA restriction endounclease fragments to high specific activity Feinberg,A.P.;B.Vogelstein
  16. Biochemistry v.13 Transcription of the genes for 5S ribosomal RNA and transfer RNA in isolated mouse myeloma cell nuclei Marzluff,W.F.;E.C.Murphy;R.C.C,Huang
  17. Proc. Natl. Acad. Sci. v.85 Insulin stimulates glycerol 3-phosphate dehydrogenase gene expression through cisacting DNA sequences Alexander,M.C.;M.Lomanto;N.Nasrin;C.Ramaika
  18. J. Biol. Chem. v.261 Transcriptional and post-transcriptional regulation of L-type pyruvate kinase gene expressiion in rat liver Vaulont,S.;A.Munnich;J.F.Decaux;A.Kahn
  19. J. Biol. Chem. v.263 Identification of e-fos sequences involved in induction by insulin and phorbol easters Stumpo,D.J.;T.N.Stewart;M.Z.Gilman;P.J.Blackshear
  20. J. Biol. Chem. v.257 Rapid changes in the concentration of phosphoe-nolpyruvate carboxykinase mRNA in rat liver and kidney Cimbala,M.A.;W.H.Lamers;K.Nelson;J.E.Monahan;H.Yoo-Warren;R.W.Hanson
  21. J. Biol. Chem. v.262 Multihormonal regulation of phosphoenolpyruvate carboxykinase-chloramphenicol acyltransferase fusion gene Magnuson,M.A.;P.G.Quinn;D.K.Granner