Streptococcus sp. 기원의 Protease를 이용한 Casein Phosphopeptides의 생산

Production of Casein Phosphopeptides by Protease from Streptococcus sp.

  • 발행 : 1993.10.01

초록

For the production of Casein Phosphopeptide(CPP) inhibiting the insolubility of calcium, 10% sodium caseinate was treated with 1.5% of protease from Streptococcus sp.. Optimal conditions and productivity for the CPP production, and properties of the CPP were compared with tryptic hydrolysates of sodium caseinate. Optimum conditions of pH, temperature and reaction time were 8.0, 50C, 4 hrs, respectively. Under these conditions the productivity of CPP was 23% and Molecular weight of CPP was ranged from 3, 000 to 17, 000. The results also showed that the insolubility of calcium was completely inhibited by using 1.5 times of CPP for the amount of calcium.

키워드

참고문헌

  1. Japan Food Sci. v.1 生理活性과 機能性食品에의 應用 濟藤安弘
  2. 한국영양학회지 v.23 영양과 관련된 질활의 현황과 대책 허갑범
  3. J. Nutr. v.104 Effect of protein intake on calcium balance of young man given 500mg calcium daily Armand,C.R.;H.M.Linkswiler
  4. Agr. Biol. Chem. v.36 vivo formation of phosphopeptide with Ca-binding property in the small intestinal tract of the rat fed on casein Naito,H.;A.Kawasaki;T.Imamura
  5. Science v.128 Calcium phosphate sequestering phosphopeptide from casein Reeves,R.E.;N.G.Latour
  6. 日本營養食科學會誌 v.39 casein의 消化時生成되는 phosphopeptide의 calcim 吸收促進機構 內藤博
  7. 食品과 開發 v.26 Peptide素材의 開發과 應用 河村幸雄
  8. Agr. Biol. Chem. v.38 Purification and some properties of an alkalophilic proteinase of a Streptomyces species Nakanish,T.;Y.Matsmura;N.Minamiura;T.Yamamoto
  9. Japan Patent 59-159792 Casein phosphopeptide production with reduced bitterness 河野海明
  10. Anal. Biochem. v.39 Molecular weight analysis of oligopeptide by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate Swank,R.W.;K.D.Munkres
  11. J. Am. Soc. v.80 The separation and amino acid composition of a pure phosphopeptone prepared from β-casein by the action of trypsin Peterson,R.F.;L.W.Nauman;T.L.Memeekeen
  12. Japan Patent 2-7616 建康飮食品 內藤博
  13. 月刊食品化學 v.6 CPP의 機能과 利用 山田昌彦
  14. 食品工業 v.33 CPP의 生理機能과 그利用 榮田利章