한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권5호
  • /
  • Pages.453-460
  • /
  • 1993
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

호알칼리성 Bacillus sp.가 생산되는 Bacteriolytic Enzyme을 이용한 Bacillus subtilis의 형질전환

Genetic Transformation of Bacillus subtilis by the Bacteriolytic Enzyme from Alkafophilic Bacillus sp.

  • 유주현 (연세대학교 식품공학과) ;
  • 이인숙 (연세대학교 식품공학과) ;
  • 옥승호 (연세대학교 식품공학과) ;
  • 박희경 (연세대학교 식품공학과) ;
  • 염도영 (연세대학교 식품공학과) ;
  • 배동훈 (단국대학교 식품공학과)
  • 발행 : 1993.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The extracellular bacteriolytic enzyme from alkalophilic Bacillus sp. YJ-451 was endopeptidase which hydrolyzes the peptide bond at the amino group of D-glutamic acid in the peptidoglycan. Protoplast transfomation system of B. subtilis by the lytic enzyme that differs, in mechanisms, from lysozyme which was used to transformation of B. subtilis was investigated. High protoplast yield was obtained from cells cultured in PAB at the late logarithmic growth phase.

키워드

참고문헌

  1. Bacteriol. Rev. v.41 Extracellular enzyme synthesis in the genus Bacillus. Priest,F.G.
  2. Bacteriol. Rev. v.21 The properties of lysozyme and its action on microorganisms Salton,M.R.J.
  3. Agr. Biol. Chem. v.45 Purification and Characterization of the lytic enzyme produced by Streptomyces rutgersensis H-46 Hayashi,K.;T.Kasumi,N.Kubo;N.Tsumura
  4. Agr. Biol. Chem. v.54 Novel bifunctional lysozyme-dextran conjugate that acts on both gram-negative and gram-positive bacteria Nakamura,S.;A.Koto;K.Kobayashi
  5. Agr. Biol. Chem. v.54 Functional protein-polysaccharide conjugate prepared by controlled dry-heating of ovalbumin-dextran mixtures high frequency transformation on Bacillus subtilis protoplast by plasmid DNA Kato,A.;Y.sasaki;R.Furuka;K.Kobayashi
  6. Kor. J. Appl. Microbiol. Bioeng. v.14 Physiological properties and transformation of alalophilic Bacillus sp. in E. coli. Yu,J.H.;Y.J.Chung;K.S.Cuung;D.H.Oh
  7. Kor. J. Appl. Microbiol. Bioeng. v.15 Molecular cloning of CMCase gene from an alalophilic Bacillus sp. in E. Coli Yu,J.H.;Y.J.Chung;J.S.Lee
  8. Kor. J. Appl. Microbiol. Bioeng. v.17 Isolation and characterization of eyelodextrin glycosyl transferase producing alkalophilic Bacillus sp. Yu,J.H.;Y.J.Chung;J.S.Lee
  9. Kor. J. Appl. Microbiol. Bioeng. v.17 Clonig and expression of β-xylosidase gene from alkali-tolerant Bacillus sp. Ya-14 Park,D.C.;J.M.Kim;Y.J.Chung;I.S.Kong;D.H.Bai;Yu,J.H.
  10. Kor. J. Appl. Microbiol. Bioeng. v.16 Cloning of pectate lyase gene of alkali-tolerant Bacillus sp. YA-14 and its expression in Escherichia coli Yu,J.H.;Y.S.Park;J.M.Kim;I.S.Kong;Y.J.Chung
  11. J. Microbiol. Biotech. v.2 Purification and characterization of a bacteriolytic enzyme from alkalophilie Bacillus sp. Jung,M.H.;I.S.Kong;D.H.Bai;J.H.Yu
  12. J. Microbiol. Biotech. v.2 Cloning and expression in E. coli of a bacteriolytic enzyme from Bacillus sp. Yu,J.H.;M.H.Jung;H.K.Park;D.H.Bai
  13. J. Microbiol. Biotech. v.3 Nucleotide sequence of a bacteriolyitic enzyme gene from akalophilic Bacillus sp. Jung,M.H.;S.H.Ohk;D.Y.Yum;I.S.Kong;D.H.Bai;J.H.Yu
  14. 석사학위논문, 연세대학교 대학원 호알칼리성 Bacillus sp.이 생산하는 용균효소에 의한 E.coli 세 포벽 용해에 관한 연구 김욱성
  15. J. Bacteriol. v.160 Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases Kawamura,F.;R.H.Doi
  16. J. Bacteriol. v.134 Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis Gryczan,T.J.;S.Contente;D.Dubnau
  17. Mol. Gen. Genet v.168 High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA Cohen,S.N.;S.Chang
  18. J. Mol. Biol. v.56 Fate of transforming DNA following uptake by competent Bacillus subtilis Dubanu,D.;R.Davidoff-Abelson
  19. J. Bacteriol. v.164 Transformal coupling in Bacillus subtilis of a heterologous Bacillus subtilis-Escherichia coli gene fusion Doi,R.H.;T.I.Zaghoul;F.Kawamura
  20. J. Bacteriol. v.137 Electron microsopic study of Bacillus subtilis protoplast fusion Frehel,C.;A.M.Lheritier;C. Sanchez-Rivas;P.Schaeffer
  21. Agric. Biol. Chem. v.46 Transformation of Bacillus protoplast by plasmid pTP4 DNA Akamatsu,T.;J.Sekiguchi
  22. Proc. Natl. Acad. Sci. v.73 Fusion of protoplasts of Bacillus megaterium Fodar,K.;L.Alf ldi
  23. Agric. Biol. Chem. v.47 Fusion of protoplasts of Streptococcus lactis Okamoto,T.;Y.Fujita;R.Irie
  24. J. Gen. Microbiol. v.127 Protoplast fusion in Streptomyces:conditions for efficient genetic recombition and cell reguration Baltz,R.H.;P.Matsushima
  25. J. Bacteriol. v.156 Genetic transformation of Bacillus brevis 47 protein-screening bacterium, by plasmid DNA Yakahashi,W.;H.Yamagata;K.Yamaguchi;N.Tsukagoshi
  26. J. Bacteriol. v.137 Parameters governing bacterial reguration and genetic recombition after fusion of Bacillus subtilis protoplant Gabor,M.H.;R.D.Hotechkiss
  27. App. Environ. Microbiol. v.48 Plasmid Transformation of Streptococcus lactis protoplasts:Optimization and use in molecular cloning Kondo,J.K.;L.L.Mckay
  28. Nature v.274 Transformation of plasmid DNA into Strepomyces at high frequency Bibb,M.J.;J.M.Ward;D.A.Hopwood
  29. J. Bacteriol. v.169 Transformation of vegatable cells of Bucillus thuringiensis by plasmid DNA Reierson,A.;R.Landen;A.L.vgren;G.Dalhammer;H.G.Boman