Abstract
Strains degrading ethylene glycol(EG) and terephthalic acid(TPA) were isolated from water systems, and identified as Pseudomonas sp. They were named as Pseudomonas sp. EAW for EG and as Pseudomonas sp. TS2 for TPA. The optimal culture conditions of temperature, pH and nitrogen source were found to be $35^{\circ}C$, 7.5 and ammonium sulfate, respectively. The growth of strains and removal efficiency was slightly promoted by trace elements such as niacin and biotin in case of EG, and by trace elements such as $Na_2MoO_4{\cdot}2H_2O$ and thiamin i case of TPA. With increasing inoculation sloe for batch culture, the removal efficiency of EG by the strain EAW was conspicuously increased, while the removal efficiency of TPA by the strain TS2 was not changed as much as that of EG. The growth rate of the strain EAW was much more decreased than that of the strain TS2 in the enrichment medium, as the frequency of repeated-batch culture in the rich-medium increased. in case of real wastewater, growth rate and removal efficiencies of EG and TPA were lower than those in the enrichment medium. $COD_{Mn}\;and\;COD_{Cr}$ removal efficiencies after 48 hrs batch culture in real wastewater were 89% and 93%, respectively. The specific growth rate was inhibited when the initial concentration of EG or TPA was more than 25g/L.
Polyester 감량폐수의 주성분인 EG,TPA분해균을 분리하여 각각 Pseudomonas sp.로 동정하여 Pseudomonas sp. EAW, Pseudomonas sp. TS2로 명명하였다. 이 두 균주의 최적 배양조건은 pH 7.5, 온도 $35^{\circ}C$ 및 질소원은 ${(NH_4)}_2SO_4$ 이며, Strain EAW의 경우 niacin과 biotin, Strain TS2의 경우 $Na_2MoO_4{\cdot}2H_2O$와 thiamine이 각 균주의 성장과 분해율에 약간씩 영향을 미치는 것으로 나타났다. Strain EAW는 접종량의 증가에 따라서 성장 및 EG의 분해시간이 현저히 감소하였으나 Strain TS2의 경우는 접종량이 증가하여도 성장 및 분해율이 큰 차이가 없었다. 또한 rich-medium에 계대배양한 각 균을 EG, T TPA액체배지에 접종하였을 때 계대배양의 회수가 증가함에 따라 Strain EAW가 Strain TS2보다 성장 및 분해율이 현저히 감소하였다. 실제 폐수에서 균들의 성장 및 분해율이 분리용 배지의 경우 보다는 약간 감소하였으며 EG, TPA 는 배양 48hr 후 $COD_{Mn}\;and\;COD_{Cr}$의 제거율은 89% 와 93%로 각각 나타났다. 회분배양시 EG,TPA의 초기농도가 25g/L 이상에서는 각 균주의 비성장속도가 감소하였다.