Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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흰쥐 태아 중뇌 배양세포에서 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine의 독성: 2',7',-Dichlorofluorescin diacetate를 이용한 연구

  • 김율아 (한림대학교 의과대학 약리학교실) ;
  • 조용준 (한림대학교 의과대학 약리학교실) ;
  • 김용식 (서울대학교 의학대학 약리학교실) ;
  • 김영희 (한림대학교 의과대학 약리학교실)
  • Published : 1993.12.01
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Abstract

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a well-known dopamine neuron-specific toxin. But the involvement of oxidative damage in the pathogenesis of MPTP-induced parkinsonism is still uncertain. In this study, by using 2',7',-dichlorofluorescin diacetate(DCFH-DA) that detects intracellular oxidative processes, the effect of MPTP on dichlorofluorescein fluorescence in dissociated cells from fetal rat mesencephalon in culture was investigated. At 7th day in culture, cells were loaded with DCFH-DA, and exposed to 1 mM MPTP or MPP+. MPTP induced dichlorofluorescein-fluorescence which was peaked at 3 min and mostly faded away 30 min after MPTP treatment.

Keywords

References

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