Ornithine Decarboxylase Activity in Porcine Reproductive Tissues

Gilt에 있어서 Tissue내에 함유되어 있는 Ornithine Decarboxylase의 활성

The tissue levels of ornithine decarboxylase (ODC) during the estrous cycle and pregnancy were investigated in the pig. Sexually mature female cycling pigs were used. One animal was sacrificed on estrous cycle days 3, 10, 17, 18, 19, 20 and during pregnancy on day 11. 12, B. 14, 18, 19, 20, 48, 50 and 52. Tissues from the hypothalamus, pituitary, uterus, ovary and skeletal muscle were removed. They were homogenized in buffer, and supernatants were used for measurement of protein concentration and ODC activity. The release of $^14$CO$_2$ from radiolabeled ornithine was proportional to the amout of protein added over the range of 0.125~4mg and to the incubation time. ODC appered to have some relationship with the biological functions of the pituitary, ovary and uterus during the reproductive period, especially on day 19 of the estrous cycle, while it showed no such activities in hypothalamus and skeletal muscle of mature pigs. Uterine tissues had significantly more ODC activity than other tissues tested(p < 0.05).

성숙한 gilt를 이용하여 estrous cycle과 pregnancyrlrks 동안 OCD효소의 tissue내 함량을 측정하는 것이 본 연구의 주 목적이었다. 하루 한 마리의 gilt를 estrous cycle day 3, 10, 17, 18, 19, 20 그리고 pregnancy day 11, 12, 13, 14, 18, 19, 20, 48, 50, 52에 도살하였다. 시상하부, 뇌하수체, 자궁, 난소 및 골겨근에서 조직을 제거하였으며, 이들 조직들을 완충액에 넣어 균질화 시켰으며 그 상청액은 단백질량과 ODC의 활력도를 측정하였는데 사용되었다. Radiolabled ormithine에서 발생하는 14CO2양은 0.124∼4mg범위내에서 단백질첨가량과 배양시간에 비례하였다. OCD는 특히 estrous cycle day 19일에 뇌하수체, 난소 그리고 자궁에서 활발한 활동을 보여주었다. 한편, 시상하부와 골격근에서 ODC는 그 어떤 활동도 보이지 않았다. 자궁조직은 다른 조직들에 비해 보다 활발한 ODC활동을 보여 주었다(P<0.05).