Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 17 Issue 3
- /
- Pages.249-255
- /
- 1993
- /
- 1226-5284(pISSN)
Nuclear Changes Occurring During Cannine Oocyte Maturation In Vitro
개 난자의 체외성숙중 핵변화
Abstract
Canine follicular oocytes were used to establish a reliable system for maturation and fertilization in vitro. Ovaries were obtained from either slaughter house or hormone-primed bitches of mixed breeds. The oocytes were recovered by mincing the ovaries in M2+BSA. Good quality of oocyte-cumulus complexes (OCCs) were selected and cultured in TCM 199 containing 15% fetal calf serum(FCS) for 24~56 h in an atmosphere of 5% CO2 at 39
Keywords