약학회지 (YAKHAK HOEJI)
- 제37권5호
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- Pages.504-510
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- 1993
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
한국인 인슈린 유전자의 클로닝 및 분석
Molecular Cloning And analysis of Korean Insulin Gene
초록
Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3'-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5'-regulatory region(G2101
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