Applied Biological Chemistry
- Volume 36 Issue 2
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- Pages.105-110
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- 1993
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Purification and properties of inulin fructotransferase (Depolymerizing) from Enterobacter sp. S45
Enterobacter sp. S45 생산 inulin fructotransferase의 정제 및 특성
- Kang, Su-Il (Institute of Agricultural science and Development, College of Agriculture and Life science, Seoul National University) ;
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Kim, Su-Il
(Department of Agricultural chemistry and Research center for New Bio-materials in Agriculture, College of Agriculture and Life science, Seoul National University)
- Published : 1993.04.30
Abstract
Inulin fructotransferase from Enterobacter sp. S45 was purified with DEAE-cellulose column chromatography and fast protein liquid chromatography. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight was estimated to be 42,800 by SDS-polyacrylamide gel electrophoresis. The optimal pH and temperature for the enzyme reaction were pH 5.5 and
Enterobacter sp. S45로부터 inulin fructotransferase를 생산, 정제하고 효소적 특성을 조사하였다. 배양상징액의
Keywords
- Inulin fructotransferase (EC 2.4.1.93);
- Enterobacter sp. S45;
- di-D-fructofuranose 1,2' : 2,3' dianhydride (DFA III)